Literature summary extracted from
Wragg, S.; Hagen, F.K.; Tabak, L.A.
Identification of essential histidine residues in UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1 (1997), Biochem. J., 328, 193-197.
No PubMed abstract available
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.1.41 |
expression of wild-type and mutants in COS7 cells |
Bos taurus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.4.1.41 |
H125A |
1% of the wild-type activity |
Bos taurus |
2.4.1.41 |
H211A |
no detectable activity |
Bos taurus |
2.4.1.41 |
H341A |
6% of the wild-type activity |
Bos taurus |
2.4.1.41 |
H344A |
no detectable activity |
Bos taurus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.1.41 |
diethyldicarbonate |
binds to His; hydroxylamine partiallly protects, UDP-GalNAc protects |
Bos taurus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.1.41 |
UDP-N-acetyl-D-galactosamine + polypeptide |
Bos taurus |
initial step of mucin-type O-glycosylation |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.41 |
Bos taurus |
- |
isozyme T1 |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.4.1.41 |
glycoprotein |
residues Asn-95 and Asn-552 of the recombinant enzyme are occupied by N-linked sugars in Cos7 cells |
Bos taurus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.41 |
UDP-N-acetyl-D-galactosamine + polypeptide |
- |
Bos taurus |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|
2.4.1.41 |
UDP-N-acetyl-D-galactosamine + polypeptide |
initial step of mucin-type O-glycosylation |
Bos taurus |
UDP + N-acetyl-D-galactosaminyl-polypeptide |
- |
? |
|