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Literature summary extracted from

  • Kamat, U.; Garg, R.; Sharma, C.B.
    Purification to homogeneity and characterization of a 1,3-beta-glucan (callose) synthase from germinating Arachis hypogaea cotyledons (1992), Arch. Biochem. Biophys., 298, 731-739.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.34 3-[(cholamidopropyl)dimethyl(ammonio)]propanesulfonate stimulate Arachis hypogaea
2.4.1.34 cellobiose no effect Arachis hypogaea
2.4.1.34 Digitonin stimulate Arachis hypogaea
2.4.1.34 GTP inhibits Arachis hypogaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.34 1,2-Bis(O-aminophenoxy)ethane-N,N,N',N'-tetraacetate
-
Arachis hypogaea
2.4.1.34 ATP
-
Arachis hypogaea
2.4.1.34 CTP
-
Arachis hypogaea
2.4.1.34 EDTA fully reversible by addition of Ca2+ Arachis hypogaea
2.4.1.34 EGTA fully reversible by addition of Ca2+ Arachis hypogaea
2.4.1.34 GTP
-
Arachis hypogaea
2.4.1.34 Nonidet P-40
-
Arachis hypogaea
2.4.1.34 octylglucoside
-
Arachis hypogaea
2.4.1.34 Triton X-100
-
Arachis hypogaea
2.4.1.34 UDP
-
Arachis hypogaea
2.4.1.34 UMP
-
Arachis hypogaea
2.4.1.34 UTP
-
Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.34 0.67
-
UDPglucose
-
Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.34 cytoplasmic membrane
-
Arachis hypogaea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.34 Ca2+ required Arachis hypogaea
2.4.1.34 Ca2+ optimum concentration 2-5 mM Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.34 48000
-
x * 48000, SDS-PAGE Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n Arachis hypogaea synthesis of callose in higher plants, enzyme is latent in intact and undamaged cells, it is activated only under perturbed conditions, possible involvement in wound-healing process and in defense against pathogens, enzyme seems to play a vital role in a number of specialized developmental processes in plants, namely pollen maturation and sieve pore formation and gravitropism UDP + (1,3-beta-D-glucosyl)n+1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.34 Arachis hypogaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.34
-
Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.34 cotyledon germinating Arachis hypogaea
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.34 -20°C, 50 mM Tris-HCl, pH 7.4, 1% digitonin, 0.01% 2-mercaptoethanol, 20% sucrose or glycerol, stable for at least 1 week, losing less than 10% of initial activity Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n specific for UDPglucose as glucosyl donor Arachis hypogaea UDP + (1,3-beta-D-glucosyl)n+1
-
?
2.4.1.34 UDPglucose + (1,3-beta-D-glucosyl)n synthesis of callose in higher plants, enzyme is latent in intact and undamaged cells, it is activated only under perturbed conditions, possible involvement in wound-healing process and in defense against pathogens, enzyme seems to play a vital role in a number of specialized developmental processes in plants, namely pollen maturation and sieve pore formation and gravitropism Arachis hypogaea UDP + (1,3-beta-D-glucosyl)n+1
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.34 ? x * 48000, SDS-PAGE Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.34 7.4
-
-
Arachis hypogaea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.34 6.5 9 6.5 and 9: about 30% of activity maximum Arachis hypogaea

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.34 ATP inhibits Arachis hypogaea