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Literature summary extracted from

  • Tachibana, Y.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Cloning and expression of the 4-alpha-glucanotransferase gene from the hyperthermophilic archaeon Pyrococcus sp. KOD1, and characterization of the enzyme (1997), J. Ferment. Bioeng., 83, 540-548.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 gene gtpK cloned, sequenced and overexpressed in Escherichia coli Pyrococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 76690
-
 calculated from cDNA Pyrococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.25 maltopentaose + maltopentaose Pyrococcus furiosus metabolism of starch in the bacterium maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose Dictyoglomus thermophilum metabolism of starch in the bacterium maltooligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Dictyoglomus thermophilum
-
-
-
2.4.1.25 Pyrococcus furiosus
-
-
-
2.4.1.25 Pyrococcus sp.
-
 KOD1
-
2.4.1.25 Pyrococcus sp. KOD1
-
 KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
 Pyrococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltohexaose + maltotriose
-
 Pyrococcus sp. maltodextrins
-
 ?
2.4.1.25 maltohexaose + maltotriose
-
 Pyrococcus sp. KOD1 maltodextrins
-
 ?
2.4.1.25 maltohexaose + maltotriose
-
 Pyrococcus sp. D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Pyrococcus furiosus maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltopentaose metabolism of starch in the bacterium Dictyoglomus thermophilum maltooligosaccharides
-
 ?
2.4.1.25 maltopentaose + maltotriose
-
 Pyrococcus sp. maltodextrins
-
 ?
2.4.1.25 maltopentaose + maltotriose
-
 Pyrococcus sp. KOD1 maltodextrins
-
 ?
2.4.1.25 maltopentaose + maltotriose
-
 Pyrococcus sp. D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltose + maltose
-
 Pyrococcus sp. maltotriose + glucose
-
 ?
2.4.1.25 maltose + maltose
-
 Pyrococcus sp. KOD1 maltotriose + glucose
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Pyrococcus sp. maltodextrins
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Pyrococcus sp. KOD1 maltodextrins
-
 ?
2.4.1.25 maltotetraose + maltotriose
-
 Pyrococcus sp. D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotheptaose + maltotriose
-
 Pyrococcus sp. maltodextrins
-
 ?
2.4.1.25 maltotheptaose + maltotriose
-
 Pyrococcus sp. KOD1 maltodextrins
-
 ?
2.4.1.25 maltotheptaose + maltotriose
-
 Pyrococcus sp. D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Pyrococcus sp. maltodextrins
-
 ?
2.4.1.25 maltotriose + maltotriose
-
 Pyrococcus sp. D-glucose + maltooligosaccharides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 100
-
-
 Pyrococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 100
-
 stable even after heating for 30 min Pyrococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6 8
-
 Pyrococcus sp.