EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.25 | gene cloned an sequenced | Dictyoglomus thermophilum |
2.4.1.25 | Gtase gene cloned and expressed in Escherichia coli MV1184 | Thermococcus litoralis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.25 | Hg2+ | - |
Thermococcus litoralis | |
2.4.1.25 | Monoiodoacetic acid | - |
Thermococcus litoralis | |
2.4.1.25 | Ni2+ | - |
Thermococcus litoralis | |
2.4.1.25 | p-chloromercuribenzoic acid | - |
Thermococcus litoralis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 77880 | - |
calculated from cDNA | Thermococcus litoralis |
2.4.1.25 | 85000 | - |
SDS-PAGE | Thermococcus litoralis |
2.4.1.25 | 87000 | - |
gel filtration | Thermococcus litoralis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.25 | Arabidopsis thaliana | - |
- |
- |
2.4.1.25 | Dictyoglomus thermophilum | - |
- |
- |
2.4.1.25 | Pyrococcus furiosus | - |
- |
- |
2.4.1.25 | Solanum tuberosum | - |
potato | - |
2.4.1.25 | Streptococcus mitis | - |
- |
- |
2.4.1.25 | Thermococcus litoralis | - |
DSM 5473 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.25 | - |
Thermococcus litoralis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.25 | leaf | - |
Solanum tuberosum | - |
2.4.1.25 | leaf | - |
Arabidopsis thaliana | - |
2.4.1.25 | tuber | - |
Solanum tuberosum | - |
2.4.1.25 | tuber | - |
Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 5.2 | - |
- |
Thermococcus litoralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.25 | amylose + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
2.4.1.25 | amylose + D-glucose | synthetic amylose AS-320 | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
2.4.1.25 | amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | cycloamylose | ? | |
2.4.1.25 | amylose + maltopentaose | - |
Thermococcus litoralis | cyclic alpha-1,4-glucan | cycloamylose | ? | |
2.4.1.25 | maltoheptaose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
2.4.1.25 | maltoheptaose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
2.4.1.25 | maltohexaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
2.4.1.25 | maltooligosaccharides | - |
Thermococcus litoralis | maltooligosaccharides + D-glucose | - |
? | |
2.4.1.25 | maltopentaose + maltopentaose | - |
Thermococcus litoralis | new oligosaccharides | - |
? | |
2.4.1.25 | maltose + maltose | - |
Thermococcus litoralis | maltotriose + glucose | - |
? | |
2.4.1.25 | maltotetraose + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
2.4.1.25 | maltotetraose + maltopentaose | - |
Arabidopsis thaliana | maltooligosaccharides | - |
? | |
2.4.1.25 | maltotetraose + maltopentaose | - |
Thermococcus litoralis | maltooligosaccharides | - |
? | |
2.4.1.25 | maltotheptaose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
2.4.1.25 | maltotriose + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + maltotetraose + maltopentaose + maltohexaose | - |
? | |
2.4.1.25 | maltotriose + maltotriose | - |
Thermococcus litoralis | maltodextrins | - |
? | |
2.4.1.25 | maltotriose + maltotriose | - |
Thermococcus litoralis | maltopentaose + maltotetraose | - |
? | |
2.4.1.25 | additional information | - |
Arabidopsis thaliana | ? | - |
? | |
2.4.1.25 | additional information | enzyme catalyzes not only intermolecular transglycosylation to produce linear alpha-1,4-glucan, but also intramolecular transglycosylation to produce cyclic alpha-1,4-glucan | Thermococcus litoralis | ? | - |
? | |
2.4.1.25 | additional information | maltose is not a product | Solanum tuberosum | ? | - |
? | |
2.4.1.25 | starch + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
2.4.1.25 | starch + D-glucose | soluble starch | Thermococcus litoralis | low molecular mass oligosaccharides | - |
? | |
2.4.1.25 | starch + glycosyl acceptor | - |
Pyrococcus furiosus | D-glucose + maltose + glucan oligosaccharides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.25 | monomer | - |
Solanum tuberosum |
2.4.1.25 | monomer | 1 * 85000, SDS-PAGE | Thermococcus litoralis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 90 | - |
- |
Thermococcus litoralis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 50 | 100 | - |
Thermococcus litoralis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 90 | - |
quite thermostable, retains full activity after 3 h | Thermococcus litoralis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 6 | - |
- |
Thermococcus litoralis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.25 | 5.5 | 10 | completely stable at 4°C for 18 h | Thermococcus litoralis |