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Literature summary extracted from

  • Jeon, B.S.; Taguchi, H.; Sakai, H.; Ohshima, T.; Wakagi, T.; Matsuzawa, H.
    4-alpha-glucanotransferase from the hyperthermophilic archaeon Thermococcus litoralis. Enzyme purification and characterization, and gene cloning, sequencing and expression in Escherichia coli (1997), Eur. J. Biochem., 248, 171-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 gene cloned an sequenced Dictyoglomus thermophilum
2.4.1.25 Gtase gene cloned and expressed in Escherichia coli MV1184 Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 Hg2+
-
Thermococcus litoralis
2.4.1.25 Monoiodoacetic acid
-
Thermococcus litoralis
2.4.1.25 Ni2+
-
Thermococcus litoralis
2.4.1.25 p-chloromercuribenzoic acid
-
Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 77880
-
calculated from cDNA Thermococcus litoralis
2.4.1.25 85000
-
SDS-PAGE Thermococcus litoralis
2.4.1.25 87000
-
gel filtration Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Arabidopsis thaliana
-
-
-
2.4.1.25 Dictyoglomus thermophilum
-
-
-
2.4.1.25 Pyrococcus furiosus
-
-
-
2.4.1.25 Solanum tuberosum
-
potato
-
2.4.1.25 Streptococcus mitis
-
-
-
2.4.1.25 Thermococcus litoralis
-
DSM 5473
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25
-
Thermococcus litoralis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.25 leaf
-
Solanum tuberosum
-
2.4.1.25 leaf
-
Arabidopsis thaliana
-
2.4.1.25 tuber
-
Solanum tuberosum
-
2.4.1.25 tuber
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 5.2
-
-
Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylose + D-glucose
-
Solanum tuberosum low molecular mass oligosaccharides
-
?
2.4.1.25 amylose + D-glucose synthetic amylose AS-320 Thermococcus litoralis low molecular mass oligosaccharides
-
?
2.4.1.25 amylose + maltopentaose
-
Solanum tuberosum cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 amylose + maltopentaose
-
Thermococcus litoralis cyclic alpha-1,4-glucan cycloamylose ?
2.4.1.25 maltoheptaose + maltopentaose
-
Arabidopsis thaliana maltooligosaccharides
-
?
2.4.1.25 maltoheptaose + maltopentaose
-
Thermococcus litoralis maltooligosaccharides
-
?
2.4.1.25 maltohexaose + maltotriose
-
Thermococcus litoralis maltodextrins
-
?
2.4.1.25 maltooligosaccharides
-
Thermococcus litoralis maltooligosaccharides + D-glucose
-
?
2.4.1.25 maltopentaose + maltopentaose
-
Thermococcus litoralis new oligosaccharides
-
?
2.4.1.25 maltose + maltose
-
Thermococcus litoralis maltotriose + glucose
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Solanum tuberosum maltooligosaccharides
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Arabidopsis thaliana maltooligosaccharides
-
?
2.4.1.25 maltotetraose + maltopentaose
-
Thermococcus litoralis maltooligosaccharides
-
?
2.4.1.25 maltotheptaose + maltotriose
-
Thermococcus litoralis maltodextrins
-
?
2.4.1.25 maltotriose + glycosyl acceptor
-
Pyrococcus furiosus D-glucose + maltose + maltotetraose + maltopentaose + maltohexaose
-
?
2.4.1.25 maltotriose + maltotriose
-
Thermococcus litoralis maltodextrins
-
?
2.4.1.25 maltotriose + maltotriose
-
Thermococcus litoralis maltopentaose + maltotetraose
-
?
2.4.1.25 additional information
-
Arabidopsis thaliana ?
-
?
2.4.1.25 additional information enzyme catalyzes not only intermolecular transglycosylation to produce linear alpha-1,4-glucan, but also intramolecular transglycosylation to produce cyclic alpha-1,4-glucan Thermococcus litoralis ?
-
?
2.4.1.25 additional information maltose is not a product Solanum tuberosum ?
-
?
2.4.1.25 starch + D-glucose
-
Solanum tuberosum low molecular mass oligosaccharides
-
?
2.4.1.25 starch + D-glucose soluble starch Thermococcus litoralis low molecular mass oligosaccharides
-
?
2.4.1.25 starch + glycosyl acceptor
-
Pyrococcus furiosus D-glucose + maltose + glucan oligosaccharides
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 monomer
-
Solanum tuberosum
2.4.1.25 monomer 1 * 85000, SDS-PAGE Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 90
-
-
Thermococcus litoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.25 50 100
-
Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 90
-
quite thermostable, retains full activity after 3 h Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6
-
-
Thermococcus litoralis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.25 5.5 10 completely stable at 4°C for 18 h Thermococcus litoralis