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Literature summary extracted from

  • Cleland, W.W.; Kennedy, E.P.
    The enzymatic synthesis of psychosine (1960), J. Biol. Chem., 235, 45-51.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.23 Tween-20 4 mg/ml, highest activity with DL-erythro-trans-sphingosine, solubilizing effect Cavia porcellus
2.4.1.23 Tween-20 4 mg/ml, highest activity with DL-erythro-trans-sphingosine, solubilizing effect Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.23 0.25
-
 UDPgalactose
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.23 microsome
-
 Cavia porcellus
-
-
2.4.1.23 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.23 Mg2+ 2-8 mM, stimulation Cavia porcellus
2.4.1.23 Mn2+ 2-3 mM, slight stimulation Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.23 Cavia porcellus
-
-
-
2.4.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.23 partial Cavia porcellus
2.4.1.23 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.23 brain
-
 Cavia porcellus
-
2.4.1.23 brain
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.23 lyophilized powder is indefinitely stable in the deep-frozen Cavia porcellus
2.4.1.23 lyophilized powder is indefinitely stable in the deep-frozen Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.23 UDPgalactose + D-1,3,4-trihydroxy-2-aminooctadecane weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-1-hydroxy-2-amino-octadecane
-
 Rattus norvegicus ?
-
 ?
2.4.1.23 UDPgalactose + DL-1-hydroxy-2-amino-octadecane weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-cis-octadecene
-
 Rattus norvegicus ?
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-cis-octadecene weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-1,3-dihydroxy-2-amino-4-heptadecyne weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-trans-sphingosine maximal activity Rattus norvegicus UDP + psychosine
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-trans-sphingosine maximal activity Cavia porcellus UDP + psychosine i. e. galactosylsphingosine ?
2.4.1.23 UDPgalactose + DL-erythro-trans-sphingosine basic structure for the acceptor seems to be a 1-hydroxy-2-amino hydrocarbon of about 18 carbon atoms Rattus norvegicus UDP + psychosine
-
 ?
2.4.1.23 UDPgalactose + DL-erythro-trans-sphingosine basic structure for the acceptor seems to be a 1-hydroxy-2-amino hydrocarbon of about 18 carbon atoms Cavia porcellus UDP + psychosine i. e. galactosylsphingosine ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-cis-octadecene weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-octadecyne
-
 Rattus norvegicus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-octadecyne weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-heptadecene weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-nonadecene weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-amino-4-trans-octadecene weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + DL-threo-1,3-dihydroxy-2-aminooctadecane weak activity Cavia porcellus ?
-
 ?
2.4.1.23 UDPgalactose + N-acetyl-DL-threo-trans-sphingosine
-
 Cavia porcellus UDP + N-acetylpsychosine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.23 37
-
 assay at Cavia porcellus
2.4.1.23 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.23 8.4
-
-
 Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.23 7 9 70% of maximal activity at pH 7, 85% of maximal activity at pH 9.0 Cavia porcellus