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Literature summary extracted from

  • Denyer, K.; Waite, D.; Motawia, S.; Moller, B.L.; Smith, A.M.
    Granule-bound starch synthase I in isolated starch granules elongates malto-oligosaccharides processively (1999), Biochem. J., 340, 183-191.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.242 additional information
-
 additional information kinetics of isozymes Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan Pisum sativum granule-bound starch synthase I synthesizes the amylose component of starch ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n Pisum sativum
-
 ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 additional information Pisum sativum isozyme GBSSI synthesizes the amylose component of starch, while isozyme GBSSII does not, due to kinetic differences ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Pisum sativum
-
-
-
2.4.1.242 Pisum sativum
-
 2 isozymes GBSSI and GBSSII in wild-type plants, isozyme GBSSI in rug5 mutant, and isozyme GBSSII in lam mutant
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.242 additional information
-
 starch synthase and malto-oligosaccharide elongation activities of isozymes, overview Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan granule-bound starch synthase I synthesizes the amylose component of starch Pisum sativum ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + maltopentaose
-
 Pisum sativum ADP + maltohexaose granule-bound starch synthase I, smaller amounts of longer malto-oligosaccharides are also produced suggesting that starch synthase I adds 1 or more glucose units to the primer ?
2.4.1.21 ADP-glucose + maltotriose
-
 Pisum sativum ADP + maltotetraose starch synthase II adds only 1 glucose unit to the primer ?
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n
-
 Pisum sativum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 additional information isozyme GBSSI synthesizes the amylose component of starch, while isozyme GBSSII does not, due to kinetic differences Pisum sativum ?
-
 ?
2.4.1.242 additional information isozyme GBSSI in isolated starch granules elongates malto-oligosaccharides progressively, adding more than one glucose molecule for each enzyme-glucan encounter, isozyme GBSSII also elongates malto-oligosaccharides but with lower affinity and not progressively Pisum sativum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.242 GBSSI
-
 Pisum sativum
2.4.1.242 granule-bound starch synthase I
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.242 25
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.242 8.5
-
 assay at Pisum sativum