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Literature summary extracted from

  • Frydman, R.B.; Cardini, C.E.
    Studies on adenosine diphosphate D-glucose:alpha-(1-4)-glucan alpha4-glucosyltransferase of sweet-corn endosperm (1965), Biochim. Biophys. Acta, 96, 294-303.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.21 adenosine 0.7 mM, 30% inhibition Zea mays subsp. mays
2.4.1.21 ADP 0.7 mM, 30% inhibition Zea mays subsp. mays
2.4.1.21 Ammonium molybdate 7 mM, complete inhibition Zea mays subsp. mays
2.4.1.21 AMP 0.7 mM, 30% inhibition Zea mays subsp. mays
2.4.1.21 ATP
-
 Zea mays subsp. mays
2.4.1.21 CaCl2 0.8 mM, 35% inhibition Zea mays subsp. mays
2.4.1.21 CoCl2 4 mM, 70% inhibition Zea mays subsp. mays
2.4.1.21 dADPglucose 1 mM, 30% inhibition Zea mays subsp. mays
2.4.1.21 HgCl2 0.3 mM, complete inhibition Zea mays subsp. mays
2.4.1.21 hydroquinone 5 mM, complete inhibition Zea mays subsp. mays
2.4.1.21 MgCl2
-
 Zea mays subsp. mays
2.4.1.21 MnCl2 7 mM, 30% inhibition Zea mays subsp. mays
2.4.1.21 p-chloromercuribenzoate 7 mM, complete inhibition Zea mays subsp. mays
2.4.1.21 ZnSO4 7 mM, complete inhibition Zea mays subsp. mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.21 additional information
-
 additional information
-
 Zea mays subsp. mays
2.4.1.21 0.75
-
 amylopectin
-
 Zea mays subsp. mays
2.4.1.21 1.1
-
 phytoglycogen
-
 Zea mays subsp. mays
2.4.1.21 16.6
-
 maltotriose
-
 Zea mays subsp. mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.21 soluble
-
 Zea mays subsp. mays
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.21 Zea mays subsp. mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.21 ammonium sulfate, DEAE-cellulose Zea mays subsp. mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.21 seed
-
 Zea mays subsp. mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.21 65.47
-
-
 Zea mays subsp. mays

Storage Stability

EC Number Storage Stability Organism
2.4.1.21 -10°C, fraction V, unstable Zea mays subsp. mays
2.4.1.21 -15°C, fraction IV, 6 months Zea mays subsp. mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.21 ADP-alpha-D-glucose + maltotriose
-
 Zea mays subsp. mays ADP + maltotetraose
-
 ?
2.4.1.21 ADP-alpha-D-glucose + phytoglycogen
-
 Zea mays subsp. mays ADP + ?
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: starch Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptors: phytoglycogen, maltotriose, maltotetraose or maltopentaose Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + alpha-1,4-polyglucan glucosyl acceptor: amylopectin Zea mays subsp. mays ADP + alpha-1,4-polyglucan
-
 ?
2.4.1.21 ADP-glucose + amylopectin
-
 Zea mays subsp. mays ADP + ?
-
 ?
2.4.1.21 dADPglucose + alpha-1,4-polyglucan 8% of activity with ADPglucose Zea mays subsp. mays dADP + alpha-1,4-polyglucan
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.21 24 30 65% activity at 37°C Zea mays subsp. mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.21 40
-
 10 min, 78% loss of activity Zea mays subsp. mays
2.4.1.21 50
-
 1 min, 75% loss of activity Zea mays subsp. mays
2.4.1.21 62
-
 inactivation at Zea mays subsp. mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.21 8.3
-
-
 Zea mays subsp. mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.21 6.5 9.7 less than half-maximal activity above and below Zea mays subsp. mays