Literature summary extracted from

Fox, J.; Kawaguchi, K.; Greenberg, E.; Preiss, J.
Biosynthesis of bacterial glycogen. Purification and properties of the Escherichia coli B ADPglucose:1,4-alpha-D-glucan 4-alpha-glucosyltransferase (1976), Biochemistry, 15, 849-856.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.1.21	D-glucono-1,5-lactone	inhibition of glycogen synthase I and II	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.1.21	additional information	-	additional information	E. coli B glycogen: 0.274 mg/ml, rabbit liver glycogen: 0.298 mg/ml, soluble potato amylose: 0.833 mg/ml	Escherichia coli
2.4.1.21	0.035	-	ADPglucose	glycogen synthases I and II	Escherichia coli

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.21	49000	-	2 * 49000, SDS-PAGE	Escherichia coli
2.4.1.21	92700	-	sucrose density gradient centrifugation	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.21	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.21	2 isozymes, DEAE-cellulose, 4-aminobutyl-Sepharose, Bio-Gel P200	Escherichia coli

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.1.21	494	-	glycogen synthase I	Escherichia coli
2.4.1.21	505	-	glcogen synthase II	Escherichia coli

Storage Stability

EC Number	Storage Stability	Organism
2.4.1.21	-85°C, at least 1 year	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.21	ADP-glucose + alpha-1,4-polyglucan	citrate independent enzyme requires a glucan primer e.g. rabbit liver glycogen	Escherichia coli	ADP + alpha-1,4-polyglucan	-	r
2.4.1.21	ADP-glucose + alpha-1,4-polyglucan	glucosyl acceptors: maltose, maltotriose, maltotetraose and maltoheptaose	Escherichia coli	ADP + alpha-1,4-polyglucan	-	r
2.4.1.21	ADP-glucose + alpha-1,4-polyglucan	glucosyl acceptor: amylose	Escherichia coli	ADP + alpha-1,4-polyglucan	-	r

Subunits

EC Number	Subunits	Comment	Organism
2.4.1.21	dimer	2 * 49000, SDS-PAGE	Escherichia coli

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.1.21	7	9.5	-	Escherichia coli

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.4.1.21	0.38	-	D-glucono-1,5-lactone	-	Escherichia coli

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Release 2023.1 (January 2023)