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Literature summary extracted from

  • Sharkey, D.J.; Kornfeld, R.
    Identification of an N-acetylglucosaminyltransferase in Dictyostelium discoideum that transfers an intersecting N-acetylglucosamine residue to high mannose oligosaccharides (1989), J. Biol. Chem., 264, 10411-10419.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.197 dithiothreitol strong inhibition in the presence of Mg2+ and Mn2+ Dictyostelium discoideum
2.4.1.197 EDTA strong Dictyostelium discoideum
2.4.1.197 EGTA strong Dictyostelium discoideum
2.4.1.197 Mg2+ strong Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.197 0.3 0.43 O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine i.e. Man5GlcNAc, depending whether N-acetyl-D-glucosamine residue is intersected or bisected Dictyostelium discoideum
2.4.1.197 0.65
-
 O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine i.e. Man9GlcNAc, intersected product Dictyostelium discoideum
2.4.1.197 1.2
-
 UDP-N-acetyl-D-glucosamine cosubstrate Man9GlcNAc Dictyostelium discoideum
2.4.1.197 1.42
-
 methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside intersected product Dictyostelium discoideum
2.4.1.197 2.54
-
 O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine bisected product Dictyostelium discoideum
2.4.1.197 18.52
-
 O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl intersected product Dictyostelium discoideum
2.4.1.197 19.48
-
 O-mannopyranosyl-alpha-1,3-mannopyranosyl-beta-1,4-N-acetyl-glucosamine bisected product Dictyostelium discoideum
2.4.1.224 0.65
-
 (mannose)9-N-acetyl-D-glucosamine
-
 Dictyostelium discoideum
2.4.1.224 1.2
-
 UDP-N-acetyl-D-glucosamine
-
 Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.197 membrane membrane-associated Dictyostelium discoideum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.197 Mn2+ stimulation Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.197 Dictyostelium discoideum
-
-
-
2.4.1.224 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.197 strain M4, partial Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.197 0.0000077
-
 Man5GlcNAc, intersected Dictyostelium discoideum
2.4.1.197 0.0000129
-
 Man9GlcNAc, intersected Dictyostelium discoideum
2.4.1.197 0.0000133
-
 Man5GlcNAc, bisected Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.197 UDP-N-acetyl-D-glucosamine + (O-mannopyranosyl-alpha-1,3)-mannopyranosyl-beta-1,4-N-acetyl-glucosamine i.e. Man2GlcNAc, very low activity Dictyostelium discoideum UDP + O-mannopyranosyl-alpha-1,3-[N-acetyl-glucosamine-beta-1,4]-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine
-
 ?
2.4.1.197 UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside trivial name Man3Me Dictyostelium discoideum UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine
-
 ?
2.4.1.197 UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine i.e. Man9GlcNAc oligosaccharide, enzyme catalyzes the transfer of N-acetyl-glucosamine to both alpha- and beta linked mannoses Dictyostelium discoideum UDP + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-(N-acetyl-D-glucosamine-beta-1,4)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine i.e. Man9GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue attached beta-1,4 to the mannose linked alpha-1,6 to the core mannose in an intersecting position ?
2.4.1.197 UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl i.e. Man2Me Dictyostelium discoideum ?
-
 ?
2.4.1.197 UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine i.e. Man5GlcNAc oligosaccharide, 20% of activity compared to Man9GlcNAc Dictyostelium discoideum UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine i.e. Man5GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue in an intersected and/or bisected position, so that either the core beta-mannose or the branching alpha-mannose residue accepts the transferred N-acetyl-D-glucosamine residue ?
2.4.1.197 UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine i.e. Man3GlcNAc Dictyostelium discoideum UDP + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6-(N-acetyl-D-glucosamine beta-1,4)]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine
-
 ?
2.4.1.224 2 UDP-N-acetyl-alpha-D-glucosamine + D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)-[D-Man-alpha-(1->3)]-D-Man-beta-(1->4)-D-GlcNAc
-
 Dictyostelium discoideum 2 UDP + D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-[D-GlcNAc-beta-(1->4)]-D-Man-alpha-(1->6)-[D-Man-alpha-(1->3)]-D-Man-beta-(1->4)-D-GlcNAc + D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)-[D-Man-alpha-(1->3)]-[D-GlcNAc-beta-(1->4)]-D-Man-beta-(1->4)-D-GlcNAc
-
 ?
2.4.1.224 UDP-N-acetyl-alpha-D-glucosamine + D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-(alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)-[D-Man-(alpha-(1->2)-D-Man-(alpha-(1->2)-D-Man-alpha-(1->3)]-D-Man-beta-(1->4)-D-GlcNAc
-
 Dictyostelium discoideum UDP D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-(alpha-(1->2)-D-Man-alpha-(1->6)]-[D-GlcNAc-beta-(1->4)]-D-Man-alpha-(1->6)-[D-Man-(alpha-(1->2)-D-Man-(alpha-(1->2)-D-Man-alpha-(1->3)]-D-Man-beta-(1->4)-D-GlcNAc
-
 ?
2.4.1.224 UDP-N-acetyl-D-glucosamine + (mannose)9-N-acetyl-D-glucosamine
-
 Dictyostelium discoideum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.197 37
-
 assay at Dictyostelium discoideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.197 45
-
 75% decrease of activity after 2 min Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.197 6 6.9
-
 Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.197 5.5 7.2 20% of maximal activity at pH 5.5, 70% of maximal activity at pH 7.2 Dictyostelium discoideum