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Literature summary extracted from

  • Paczkowski, C.; Wojciechowski, Z.A.
    The occurence of UDPG-dependent glucosyltransferase specific for sarsasapogenin in Asparagus officinalis (1988), Phytochemistry, 27, 2743-2747.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.193 EDTA 1 mM, 43% inhibition Asparagus officinalis
2.4.1.193 EGTA 10 mM, 50% inhibition Asparagus officinalis
2.4.1.193 PCMB 0.01 mM, 94% inhibition Asparagus officinalis
2.4.1.193 Triton X-100
-
 Asparagus officinalis
2.4.1.193 UDP 1 mM, 89% inhibition Asparagus officinalis
2.4.1.193 UMP 1 mM, 51% inhibition Asparagus officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.193 cytosol
-
 Asparagus officinalis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.193 Mg2+ 1 mM, stimulates Asparagus officinalis
2.4.1.193 Mn2+ 1 mM, stimulates Asparagus officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.193 UDPglucose + (25S)-5beta-spirostan-3beta-ol Asparagus officinalis involved in the biosynthesis of plant saponins UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.193 Asparagus officinalis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.193 shoot of 3-week-old plants Asparagus officinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.193 UDPglucose + (25R)-5alpha-spirostan-3beta-ol weak activity Asparagus officinalis UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25R)-5alpha-spirostan-3beta-ol-12-one weak activity Asparagus officinalis UDP + (25R)-5alpha-spirostan-3beta-ol-12-one 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25R)-5alpha-spirostane-3beta,6alpha-diol weak activity Asparagus officinalis UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25R)-5beta-spirostan-3beta-ol i.e. smilagenin Asparagus officinalis UDP + (25R)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25R)-spirost-5-en-3beta-ol weak activity Asparagus officinalis UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25S)-5beta-spirostan-3beta-ol i.e. sarsapogenin Asparagus officinalis UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + (25S)-5beta-spirostan-3beta-ol involved in the biosynthesis of plant saponins Asparagus officinalis UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + 5alpha-cholestan-3beta-ol weak activity Asparagus officinalis UDP + 5alpha-cholestan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + 5beta-cholestan-3beta-ol weak activity Asparagus officinalis UDP + 5beta-cholestan-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.193 UDPglucose + stigmast-5-en-3beta-ol weak activity Asparagus officinalis UDP + stigmast-5-en-3beta-ol 3-O-beta-D-glucoside
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.193 9.6
-
-
 Asparagus officinalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.193 7.2 9.9 pH 7.2: about 40% of maximal activity, pH 9.9: about 60% of maximal activity Asparagus officinalis