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Literature summary extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Subcellular localization of UDPG:nuatigenin glucosyltransferase in oat leaves (1987), Phytochemistry, 26, 353-357.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 Triton X-100 activity stimulated at 0.3% Avena sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.192 Hg2+
-
 Avena sativa
2.4.1.192 Triton X-100
-
 Avena sativa
2.4.1.192 Zn2+
-
 Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 membrane
-
 Avena sativa 16020
-
2.4.1.192 cytosol 70% of the activity Avena sativa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.192 additional information no stimulation by divalent cations: Ca2+, Mn2+ or Mg2+ Avena sativa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Avena sativa
-
-
-
2.4.1.192 Avena sativa
-
 oat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial purification by differential centrifugation Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 leaf
-
 Avena sativa
-
2.4.1.173 root
-
 Avena sativa
-
2.4.1.192 leaf
-
 Avena sativa
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.192 -20°C, cytosolic fraction stable for several months Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 UDP-glucose + sitosterol
-
 Avena sativa UDP + sitosterol 3-beta-D-glucoside
-
 ?
2.4.1.192 (25R)-spirost-5-en-3beta-ol + UDP-glucose
-
 Avena sativa UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
-
 ?
2.4.1.192 20S,22S,25S-spirost-5-ene-3beta,25-diol + UDP-glucose
-
 Avena sativa UDP + 20S,22S,25S-spirost-5-ene-3beta,25-diol 3-O-beta-D-glucoside
-
 ?
2.4.1.192 UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol
-
 Avena sativa UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside i.e. nuatigenin 3beta-D-monoglucoside ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.173 30
-
 assay at Avena sativa
2.4.1.192 30
-
-
 Avena sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.192 30 39 at 30°C activity maximum, at 39°C activity is 7times lower than at 30°C Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.173 7.5
-
 assay at Avena sativa
2.4.1.192 6.5 8.5
-
 Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.192 5.8 8.5 at pH 5.8 about 50% of activity maximum, at pH 6.5-8.5 activity maximum Avena sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.173 7.5
-
 Tris-HCl 0.1 M Avena sativa