Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujita, Y.; Tsubouchi, H.; Inagi, Y.; Tomita, K.; Ozaki, A.; Nakanishi, K.
    Purification and properties of cyclodextrin glycosyltransferase from Bacillus sp. AL-6 (1990), J. Ferment. Bioeng., 70, 150-154.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.19 EDTA 80 mM, relative activity 117% Bacillus sp. (in: Bacteria)
2.4.1.19 ethanol
-
 Niallia circulans
2.4.1.19 ethanol
-
 Paenibacillus macerans
2.4.1.19 ethanol enhances the formaton of gamma-cyclodextrin Bacillus sp. (in: Bacteria)
2.4.1.19 p-chloromercuribenzoic acid 80 mM, relative activity 118% Bacillus sp. (in: Bacteria)
2.4.1.19 phenylmethylsulfonyl fluoride 80 mM, relative activity 114% Bacillus sp. (in: Bacteria)

General Stability

EC Number General Stability Organism
2.4.1.19 relatively stable to Hg2+ Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 (NH4)6Mo7O24
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 Cu2+ CuSO4 Bacillus sp. (in: Bacteria)
2.4.1.19 Fe2+ FeSO4 Bacillus sp. (in: Bacteria)
2.4.1.19 Mo6+
-
 Bacillus sp. (in: Bacteria)
2.4.1.19 Zn2+ ZnSO4 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 Ca2+
-
 Niallia circulans
2.4.1.19 Ca2+
-
 Paenibacillus macerans
2.4.1.19 Ca2+ 4 mM CaCl2, relative activity 106% Bacillus sp. (in: Bacteria)
2.4.1.19 K+ 80 mM KCl, relative activity 112% Bacillus sp. (in: Bacteria)
2.4.1.19 Na+ 80 mM NaCl, relative activity 110% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 74000
-
-
 Paenibacillus macerans
2.4.1.19 74000
-
 gel filtration, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor Paenibacillus macerans
-
 Schardinger dextrins
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. (in: Bacteria)
-
 AL-6
-
2.4.1.19 Bacillus sp. (in: Bacteria) AL-6
-
 AL-6
-
2.4.1.19 Bacillus subtilis
-
 no.313
-
2.4.1.19 Bacillus subtilis 313
-
 no.313
-
2.4.1.19 Niallia circulans
-
 B. sp. var. alkalophilus No. 38-2, ATCC 21783
-
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.19 877.9
-
 strain Al-6 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 potato starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) gamma-cyclodextrin + beta-cyclodextrin
-
 r
2.4.1.19 potato starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 gamma-cyclodextrin + beta-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans Schardinger dextrins
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) beta-cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Niallia circulans beta-cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 beta-cyclodextrin + gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor strain no.313 Bacillus subtilis gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor
-
 Bacillus sp. (in: Bacteria) AL-6 gamma-cyclodextrin
-
 r
2.4.1.19 soluble starch + glycosyl acceptor strain no.313 Bacillus subtilis 313 gamma-cyclodextrin
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.19 monomer
-
 Niallia circulans
2.4.1.19 monomer
-
 Paenibacillus macerans
2.4.1.19 monomer 1 * 74000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 55
-
-
 Priestia megaterium
2.4.1.19 55
-
-
 Niallia circulans
2.4.1.19 55
-
-
 Paenibacillus macerans
2.4.1.19 55
-
 strain AL-6, starch dextrinizing Bacillus sp. (in: Bacteria)
2.4.1.19 60
-
-
 Niallia circulans
2.4.1.19 60
-
-
 Paenibacillus macerans
2.4.1.19 60
-
 strain AL-6, cyclodextrin forming Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 40
-
 quite stable at pH 5.0-8.0 for 3 h Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 7 10 strain AL-6 Bacillus sp. (in: Bacteria)
2.4.1.19 7.5 10.5 strain AL-6, cyclodextrin forming Bacillus sp. (in: Bacteria)
2.4.1.19 8
-
-
 Bacillus subtilis
2.4.1.19 8
-
 strain AL-6, starch dextrinizing Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.19 5 8
-
 Paenibacillus macerans
2.4.1.19 5 8 AL-6, quite stable at 40°C for 3 h Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 5 8 quite stable at 40°C for 3 h Bacillus sp. (in: Bacteria)