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Literature summary extracted from

  • Schulz, M.; Weissenböck, G.
    Three specific UDP-glucuronate: flavone-glucuronosyl-transferases from primary leaves of Secale cereale (1988), Phytochemistry, 27, 1261-1267.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.189 freezing without glycerol, complete inactivation Secale cereale
2.4.1.190 freezing without glycerol, complete inactivation Secale cereale
2.4.1.191 freezing without glycerol, complete loss of activity Secale cereale

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.189 luteolin 0.14 mM, 60% inhibition Secale cereale
2.4.1.189 UDP strong inhibition Secale cereale
2.4.1.190 Ag+ 0.75 mM, 40% inhibition Secale cereale
2.4.1.190 Cu2+ 0.75 mM, 40% inhibition Secale cereale
2.4.1.190 luteolin 0.1 mM, 50% inhibition Secale cereale
2.4.1.190 Pb2+ 0.75 mM, 40% inhibition Secale cereale
2.4.1.190 UDP
-
 Secale cereale
2.4.1.191 luteolin 0.02 mM, 50% inhibition Secale cereale
2.4.1.191 UDP strong Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.189 0.008
-
 luteolin
-
 Secale cereale
2.4.1.189 0.012
-
 UDPglucuronic acid
-
 Secale cereale
2.4.1.190 0.012
-
 luteolin 7-O-glucuronide
-
 Secale cereale
2.4.1.190 0.04
-
 UDPglucuronic acid
-
 Secale cereale
2.4.1.191 0.009
-
 Luteolin 7-O-diglucuronide
-
 Secale cereale
2.4.1.191 0.09
-
 UDPglucuronic acid
-
 Secale cereale

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.189 cytosol probably Secale cereale 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.189 Ca2+ 0.25 mM, 25% stimulation Secale cereale
2.4.1.189 Mg2+ up to 1 mM, 20% stimulation Secale cereale
2.4.1.191 additional information not affected by Ca2+ or Mg2+ up to 0.5 and 0.75 mM Secale cereale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.189 34500
-
 gel filtration Secale cereale
2.4.1.190 37000
-
 gel filtration Secale cereale
2.4.1.191 29000
-
 gel filtration Secale cereale

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.189 UDPglucuronic acid + luteolin Secale cereale first enzyme in the biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-glucuronide
-
 ir
2.4.1.190 UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide Secale cereale involved in the sequential biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
2.4.1.191 UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] Secale cereale involved in the sequential biosynthesis of luteolin triglucuronide UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.189 Secale cereale
-
 rye
-
2.4.1.190 Secale cereale
-
 rye
-
2.4.1.191 Secale cereale
-
 rye
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.189 ultrogel AcA 44, DEAE-cellulose, partial purification Secale cereale
2.4.1.190 Ultrogel AcA 44, ultrafiltration, hydroxylapatite, partial purification Secale cereale
2.4.1.191 Ultrogel AcA 44, Sephadex G-25, ultrafiltration, partial purification Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.189 leaf
-
 Secale cereale
-
2.4.1.190 leaf
-
 Secale cereale
-
2.4.1.191 leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.189 1.16
-
-
 Secale cereale
2.4.1.190 0.96
-
-
 Secale cereale
2.4.1.191 0.14
-
-
 Secale cereale

Storage Stability

EC Number Storage Stability Organism
2.4.1.189 -20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale
2.4.1.190 -20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale
2.4.1.191 -20°C, 50% glycerol, 3 months, 30-50% loss of activity Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.189 UDP-alpha-D-glucose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-beta-D-glucoside
-
 ?
2.4.1.189 UDPgalactose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-galactoside
-
 ?
2.4.1.189 UDPglucuronic acid + apigenin 38% of activity compared to luteolin Secale cereale UDP + apigenin 7-O-glucuronide
-
 ?
2.4.1.189 UDPglucuronic acid + luteolin
-
 Secale cereale UDP + luteolin 7-O-glucuronide
-
 ir
2.4.1.189 UDPglucuronic acid + luteolin first enzyme in the biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-glucuronide
-
 ir
2.4.1.189 UDPxylose + luteolin 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-xyloside
-
 ?
2.4.1.190 UDP-glucuronic acid + apigenin 7-O-beta-D-glucuronide 88% of activity with luteolin 7-O-glucuronide Secale cereale UDP + apigenin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
2.4.1.190 UDP-glucuronic acid + chrysoeriol 7-O-beta-D-glucuronide 65% of activity with luteolin-7-O-glucuronide Secale cereale UDP + chrysoeriol 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
2.4.1.190 UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide
-
 Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ir
2.4.1.190 UDP-glucuronic acid + luteolin 7-O-beta-D-glucuronide involved in the sequential biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 ?
2.4.1.190 UDPglucuronic acid + apigenin 7-O-glucoside 51.5% of activity with luteolin 7-O-glucuronide Secale cereale UDP + apigenin-7-O-beta-D-glucosyl-1,4-beta-D-glucuronoside
-
 ?
2.4.1.191 UDP-galactose + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide] 7.5% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-galactoside
-
 ?
2.4.1.191 UDP-glucose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucoside
-
 ?
2.4.1.191 UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]
-
 Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir
2.4.1.191 UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] involved in the sequential biosynthesis of luteolin triglucuronide Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide
-
 ir
2.4.1.191 UDP-glucuronic acid + luteolin-7-O-glucuronide
-
 Secale cereale UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide
-
 ?
2.4.1.191 UDP-xylose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] 10% of activity with UDPglucuronic acid Secale cereale UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-xyloside
-
 ?
2.4.1.191 UDPglucuronic acid + luteolin-7-O-glucuronide 34% of activity with luteolin 7-O-diglucuronide Secale cereale UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.189 50
-
-
 Secale cereale
2.4.1.190 52
-
-
 Secale cereale
2.4.1.191 40
-
-
 Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.189 6.5
-
 50% of maximal activity at pH 5.6 Secale cereale
2.4.1.190 6.5
-
-
 Secale cereale
2.4.1.190 8.5
-
 pH-optimum at pH 8.5 is absent in crude enzyme preparations Secale cereale
2.4.1.191 7
-
-
 Secale cereale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.189 5.6 9 50% of maximal activity at pH 5.6 and pH 9.0 Secale cereale
2.4.1.190 6 9 50% of maximal activity at pH 6.0 and pH 9.0 Secale cereale
2.4.1.191 6 9 50% of maximal activity at pH 6 and pH 9 Secale cereale

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.189 0.26
-
 UDP
-
 Secale cereale
2.4.1.190 0.12
-
 UDP
-
 Secale cereale