Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Murazumi, N.; Kumita, K.; Araki, Y.; Ito, E.
    Partial purification and properties of UDP-N-acetylmannosamine:N-acetylglucosaminyl pyrophosphorylundecaprenol N-acetylmannosaminyltransferase from Bacillus subtilis (1988), J. Biochem., 104, 980-984.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.187 glycerol 25%: twofold increase of activity Bacillus subtilis
2.4.1.187 Nonidet P-40 0.1%: small stimulatory effect Bacillus subtilis

General Stability

EC Number General Stability Organism
2.4.1.187 glycerol, 25%, stabilizes Bacillus subtilis
2.4.1.187 Nonidet P-40, 0.1-0.2%, stabilizes Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.187 Triton X-100 strong Bacillus subtilis
2.4.1.187 UDP 2 mM, strong Bacillus subtilis
2.4.1.187 UMP weak Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.187 0.0044
-
 UDP-N-acetylmannosamine
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.187 (NH4)2SO4 0.3-0.5 M: stimulation Bacillus subtilis
2.4.1.187 KCl 0.3 M: stimulation Bacillus subtilis
2.4.1.187 MgCl2 10 mM, highest stimulatory effect Bacillus subtilis
2.4.1.187 NaCl 0.3-0.5 M: stimulation Bacillus subtilis
2.4.1.187 NH4Cl 0.3-0.5 M: stimulation Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.187 additional information Bacillus subtilis involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls ?
-
 ?
2.4.1.187 additional information Bacillus subtilis AHU 1035 involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls ?
-
 ?
2.4.1.187 UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol Bacillus subtilis
-
 UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?
2.4.1.187 UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol Bacillus subtilis AHU 1035
-
 UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.187 Bacillus subtilis
-
 AHU 1035
-
2.4.1.187 Bacillus subtilis AHU 1035
-
 AHU 1035
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.187 partial Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.187 0.0000022
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.4.1.187 -18°C, several months, no significant loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.187 additional information involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls Bacillus subtilis ?
-
 ?
2.4.1.187 additional information involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls Bacillus subtilis AHU 1035 ?
-
 ?
2.4.1.187 UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
-
 Bacillus subtilis UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?
2.4.1.187 UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
-
 Bacillus subtilis AHU 1035 UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.187 25
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.187 7.3
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.187 6 9 40% of maximal activity at pH 6, 45% of maximal activity at pH 9 Bacillus subtilis