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Literature summary extracted from

  • McIntosh, C.A.; Latchinian, L.; Mansell, R.L.
    Flavanone-specific 7-O-glucosyltransferase activity in Citrus paradisi seedlings: purification and characterization (1990), Arch. Biochem. Biophys., 282, 50-57.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.185 0.063
-
 naringenin
-
 Citrus x paradisi
2.4.1.185 0.124
-
 hesperetin
-
 Citrus x paradisi
2.4.1.185 0.243
-
 UDPglucose
-
 Citrus x paradisi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.185 54900
-
 gel filtration Citrus x paradisi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone Citrus x paradisi enzyme does not accept other flavanone or flavonol aglycones UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside trivial name prunin ?
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone Citrus x paradisi trivial name naringenin UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside trivial name prunin ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.185 Citrus x paradisi
-
 grapefruit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.185 ammonium sulfate, Sephadex G-100, hydroxyapatite, UDP-glucuronic acid agarose, Mono Q, Mono P Citrus x paradisi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.185 leaf
-
 Citrus x paradisi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.185 0.0017
-
-
 Citrus x paradisi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone enzyme does not accept other flavanone or flavonol aglycones Citrus x paradisi UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside trivial name prunin ?
2.4.1.185 UDP-alpha-D-glucose + 4',5',7-trihydroxyflavanone trivial name naringenin Citrus x paradisi UDP + 4',5',7-trihydroxyflavanone 7-O-beta-D-glucoside trivial name prunin ?
2.4.1.185 UDP-glucose + hesperetin trivial name hesperetin, 83% of activity with naringenin Citrus x paradisi UDP + hesperetin 7-O-glucoside
-
 ?
2.4.1.185 UDP-glucose + naringenin
-
 Citrus x paradisi UDP + naringenin 7-O-glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.185 30
-
 assay at Citrus x paradisi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.185 7.5
-
 naringenin Citrus x paradisi
2.4.1.185 7.5 8 hesperetin Citrus x paradisi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.185 5.5 7.5 60% of maximal activity at pH 5.5 and pH 7.5 Citrus x paradisi