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Literature summary extracted from

  • Heemskerk, J.W.M.; Jacobs, F.H.H.; Scheijen, M.A.M.; Helsper, J.P.F.G.; Wintermans, J.F.G.M.
    Characterization of galactosyltransferases in spinach chloroplast envelopes (1987), Biochim. Biophys. Acta, 918, 189-203.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.184 NH4+ 50 mM, 5fold stimulation Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.46 4-hydroxymercuribenzoic acid 0.01 mM, 65% inhibition Spinacia oleracea
2.4.1.46 Cd2+
-
 Spinacia oleracea
2.4.1.46 N-ethylmaleimide 0.005-0.1 mM, complete inhibition of solubilized enzyme; 0.5 mM, 57% inhibition Spinacia oleracea
2.4.1.46 oleic acid 0.3 mM, 58% inhibition Spinacia oleracea
2.4.1.46 UDP 0.15 mM, 79% inhibition Spinacia oleracea
2.4.1.46 Zn2+ 10 mM, complete inhibition, stimulation at 1 mM Spinacia oleracea
2.4.1.184 CdCl2 2 mM, 50% inhibition Spinacia oleracea
2.4.1.184 N-ethylmaleimide
-
 Spinacia oleracea
2.4.1.184 p-hydroxymercuribenzoate
-
 Spinacia oleracea
2.4.1.184 UDP 8 mM, 50% inhibition Spinacia oleracea
2.4.1.184 UMP 10 mM, 50% inhibition Spinacia oleracea
2.4.1.184 Zn2+ 1 mM, 75% inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.46 0.04
-
 UDPgalactose
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 chloroplast envelope
-
 Spinacia oleracea 9941
-
2.4.1.184 chloroplast envelope envelope Spinacia oleracea 9941
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.46 Mg2+ stimulates Spinacia oleracea
2.4.1.46 Mg2+ cation not required Spinacia oleracea
2.4.1.46 Mn2+ stimulates, cation not required Spinacia oleracea
2.4.1.46 Zn2+ 1 mM, stimulation, inhibition at 10 mM Spinacia oleracea
2.4.1.184 Ba2+ 10 mM, 23fold stimulation Spinacia oleracea
2.4.1.184 Ca2+ 10 mM, 23fold stimulation Spinacia oleracea
2.4.1.184 Co2+ 10 mM, 3fold simulation Spinacia oleracea
2.4.1.184 Fe2+ 10 mM, 6fold simulation Spinacia oleracea
2.4.1.184 K+ 50 mM, 5fold stimulation Spinacia oleracea
2.4.1.184 Mg2+ 10 mM, 20fold stimulation Spinacia oleracea
2.4.1.184 Mn2+ 10 mM, 23fold stimulation Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Spinacia oleracea
-
-
-
2.4.1.184 Spinacia oleracea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-dioleoylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-dioleoylglycerol
-
 ?
2.4.1.46 UDP-galactose + 1,2-distearoylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-distearoylglycerol
-
 ?
2.4.1.184 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol digalactosyldiacylglycerol synthesis proceeds most rapidly by galactosyl transfer to hexaene species of monogalactosyldiacylglycerol Spinacia oleracea 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-galactosyl]-sn-glycerol + 1,2-diacyl-sn-glycerol by further transfer of galactosyl residues to the digalactosyldiacylglycerol, trigalactosyldiacylglycerol and tetragalactosyldiacylglycerol are also formed ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.46 30
-
 assay at, activity maximum in long-term incubation Spinacia oleracea
2.4.1.46 50
-
 activity maximum in short-term incubation Spinacia oleracea
2.4.1.184 30
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.46 7
-
 approx. value Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.46 5.5 9 55% of maximal activity at pH 5.5, 85% of maximal activity at pH 9.0 Spinacia oleracea