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Literature summary extracted from

  • Seo, H.S.; Koo, Y.J.; Lim, J.Y.; Song, J.T.; Kim, C.H.; Kim, J.K.; Lee, J.S.; Choi, Y.D.
    Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli (2000), Appl. Environ. Microbiol., 66, 2484-2490.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 construction of a bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase and expression in Escherichia coli Escherichia coli
3.1.3.12 the chimeric gene is expressed in Escherichia coli Escherichia coli
3.1.3.12 the chimeric gene is expressed in Escherichia coli, a bifunctional fusion enzyme is constructed by fusing genes for trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, this enzyme catalysing sequential reactions has kinetic advantages over a mixture of both enzymes Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.15 additional information
-
 additional information bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 3.7
-
 glucose-6-phosphate
-
 Escherichia coli
2.4.1.15 5.1
-
 UDPglucose bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 7.5
-
 glucose 6-phosphate bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 8.6
-
 UDPglucose
-
 Escherichia coli
3.1.3.12 2.5
-
 trehalose 6-phosphate pH 7.5, 30°C Escherichia coli
3.1.3.12 2.6
-
 trehalose 6-phosphate pH 7.5, 30°C, chimeric enzyme Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.12 37400
-
 SDS-PAGE Escherichia coli
3.1.3.12 88300
-
 chimeric enzyme which contains trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.12 trehalose 6-phosphate + H2O Escherichia coli
-
 trehalose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Escherichia coli
-
-
-
3.1.3.12 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.12 additional information a bifunctional fusion enzyme is constructed by fusing genes for trehalose 6-phosphate synthetase and trehalose 6-phosphate phosphatase, this enzyme catalysing sequential reactions has kinetic advantages over a mixture of both enzymes Escherichia coli

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
3.1.3.12 the chimeric gene Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 UDPglucose + glucose 6-phosphate
-
 Escherichia coli trehalose 6-phosphate + UDP
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Escherichia coli trehalose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.12 monomer 1 * 37400, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.15 30 40 bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
3.1.3.12 30 40 both the chimeric enzyme and the equimolar mixture of trehalose 6-phosphate synthetase/trehalose 6-phosphate phosphatase Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.12 50
-
 the termal stability of chimeric enzyme and equimolare mixture are quite similar Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.15 additional information
-
 additional information bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 3.2
-
 glucose 6-phosphate bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 3.6
-
 UDPglucose bifunctional fusion enzyme of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase Escherichia coli
2.4.1.15 6.7
-
 glucose 6-phosphate
-
 Escherichia coli
2.4.1.15 7.3
-
 UDPglucose
-
 Escherichia coli
3.1.3.12 14.3
-
 trehalose 6-phosphate pH 7.5, 30°C Escherichia coli
3.1.3.12 14.6
-
 trehalose 6-phosphate pH 7.5, 30°C, chimeric enzyme Escherichia coli