Literature summary extracted from

Pan, Y.T.; Mitchell, M.; Elbein, A.D.
Studies on the trehalose-phosphate synthase of Mycobacterium smegmatis: binding of heparin to the enzyme (1978), Arch. Biochem. Biophys., 186, 392-400.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.4.1.15	heparin	activation	Mycolicibacterium smegmatis
2.4.1.15	Triton X-100	activation	Mycolicibacterium smegmatis
2.4.1.15	Tween 80	activation	Mycolicibacterium smegmatis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.1.15	(NH4)2SO4	1 mM	Mycolicibacterium smegmatis
2.4.1.15	deoxycholate	-	Mycolicibacterium smegmatis
2.4.1.15	NaCl	25 mM or higher	Mycolicibacterium smegmatis
2.4.1.15	phosphate	0.1 M	Mycolicibacterium smegmatis
2.4.1.15	SDS	-	Mycolicibacterium smegmatis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.1.15	1	-	glucose 6-phosphate	reaction with GDPglucose	Mycolicibacterium smegmatis
2.4.1.15	1.2	-	glucose 6-phosphate	reaction with UDPglucose	Mycolicibacterium smegmatis
2.4.1.15	1.6	-	UDPglucose	-	Mycolicibacterium smegmatis
2.4.1.15	2	-	glucose 6-phosphate	GDPglucose	Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.15	45000	-	gel filtration	Mycolicibacterium smegmatis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.15	Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.15	-	Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.1.15	additional information	-	-	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.15	UDPglucose + glucose 6-phosphate	-	Mycolicibacterium smegmatis	trehalose 6-phosphate + UDP	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.4.1.15	?	x * 45000, aggregation to dimer or oligomer, SDS-PAGE	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)