Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schilling, N.
    Characterization of maltose biosynthesis from alpha-D-gluccose-1-phosphate in Spinacia oleracea L. (1982), Planta, 154, 87-93.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.139 during purification addition of 15 mM alpha-D-glucose 1-phosphate to the isolation buffer is necessary for stabilization Spinacia oleracea
2.4.1.139 purification has to be carried out at 4°C Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.139 ADPglucose strong Spinacia oleracea
2.4.1.139 ATP
-
 Spinacia oleracea
2.4.1.139 beta-D-glucose 1-phosphate
-
 Spinacia oleracea
2.4.1.139 D-glucono-1,5-lactone competitive Spinacia oleracea
2.4.1.139 GDPglucose strong Spinacia oleracea
2.4.1.139 phosphate
-
 Spinacia oleracea
2.4.1.139 UDPglucose strong Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.139 1.5
-
 alpha-D-glucose 1-phosphate
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.139 95000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.139 alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate Spinacia oleracea
-
 maltose + phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.139 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.139 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.139 cotyledon
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.139 additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.139 alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate
-
 Spinacia oleracea maltose + phosphate + phosphate
-
 ?
2.4.1.139 additional information exchange of phosphate group of alpha-D-glucose 1-phosphate with phosphate is possible, neither maltose 1-phosphate nor free phosphate can be detected as an intermediate Spinacia oleracea ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.139 6.8
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.139 6.2 7.5 30% of maximal activity at pH 6.2, 45% of maximal activity at pH 7.5 Spinacia oleracea