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Literature summary extracted from

  • Sharma, C.B.; Lehle, L.; Tanner, W.
    N-Glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyl transferase (1981), Eur. J. Biochem., 116, 101-108.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.99.18 glycerol, 25%, stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.18 octylglucoside concentration 1% Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.18 0.0005
-
 dolichyl diphosphochitobiose-(Man)9-(Glc)3
-
 Saccharomyces cerevisiae
2.4.99.18 0.0012
-
 dolichyl diphosphochitobiose
-
 Saccharomyces cerevisiae
2.4.99.18 0.05
-
 Tyr-Asn-Leu-Thr-Ser-Val reaction with dolichyl diphosphochitobiose-Man9Glc3 Saccharomyces cerevisiae
2.4.99.18 0.6
-
 Tyr-Asn-Leu-Thr-Ser-Val reaction with dolichyl diphosphochitobiose Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.18 membrane bound to Saccharomyces cerevisiae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.18 Mg2+ divalent cation required. Mg2+, at concentration of 10 mM yields only one third the activity observed with Mn2+ Saccharomyces cerevisiae
2.4.99.18 Mn2+ divalent cation required, 1 mM Mn2+ is optimal Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Saccharomyces cerevisiae
-
 wild-type strain X-2180-1A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.18 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.99.18 additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.4.99.18 -20°C, Nonidet-solubilized crude enzyme, 25% glycerol, 0.01% 2-mercaptoethanol, 18% loss of activity after 1 month and 87% after 5 months Saccharomyces cerevisiae
2.4.99.18 -70°C, Nonidet-solubilized crude enzyme, 25% glycerol, 0.01% 2-mercaptoethanol, retains 90% of original activity after 1 month and 55% after 5 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 dolichyl diphosphate-di-N-acetylchitobiose + Tyr-Asn-Leu-Thr-Ser-Val
-
 Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 dolichyl diphosphochitobiose-(mannosyl)9-(glucosyl)3 + synthetic hexapeptide glycosyl-donors are also dolichyl diphosphochitobiose or dolichyl diphospho-chitobiose-mannose, no substrates are dolichyl diphosphochitobiose-(mannosyl)9 or dolichyl diphospho-N-acetyl-D-glucosamine Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 dolichyl diphosphochitobiose-(mannosyl)9-(glucosyl)3 + synthetic hexapeptide e.g. Tyr-Asn-Leu-Thr-Ser-Val Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine glycosyl donor specificity Saccharomyces cerevisiae dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl-diphosphochitobiose + Tyr-Asn-Leu-Thr-Ser-Val
-
 Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 dolichyl-diphosphochitobiose-Man1 + Tyr-Asn-Leu-Thr-Ser-Val
-
 Saccharomyces cerevisiae ?
-
 ?
2.4.99.18 dolichyl-diphosphochitobiose-Man9Glc3 + Tyr-Asn-Leu-Thr-Ser-Val
-
 Saccharomyces cerevisiae ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.18 6.5 7.7
-
 Saccharomyces cerevisiae