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Literature summary extracted from

  • Imperiali, B.; Shannon, K.L.
    Differences between Asn-Xaa-Thr-containing peptides: a comparison of solution conformation and substrate behavior with oligosaccharyltransferase (1991), Biochemistry, 30, 4374-4380.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.18 0.25
-
 benzoyl-Asn-Leu-Thr-N-methyl-threonine
-
 Sus scrofa
2.4.99.18 1
-
 Ac-Asn-Leu-Thr-NH2
-
 Sus scrofa
2.4.99.18 2
-
 Ac-Asn-Ala-Thr-NH2
-
 Sus scrofa
2.4.99.18 3.3
-
 Asn-Asp-Thr
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.18 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.99.18 liver
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
2.4.99.18 -85°C, membrane preparation, 30% glycerol, several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.18 dolichyl diphosphooligosaccharide + Ac-Asn-Ala-Thr-NH2
-
 Sus scrofa ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + Ac-Asn-Leu-Thr-NH2
-
 Sus scrofa ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + Asn-Asp-Thr
-
 Sus scrofa ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + benzoyl-Asn-Leu-Thr-N-methyl-threonine
-
 Sus scrofa ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + protein L-asparagine peptide acceptor specificity Sus scrofa dolichyl diphosphate + glycoprotein with the oligosaccharide chain attached by N-glycosyl-linkage to protein L-asparagine
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + synthetic tripeptides Ac-Asn-Leu-Thr-NH2, Ac-Asn-Ala-Thr-NH2 and benzoyl-Asn-Leu-N-methylamide Sus scrofa ?
-
 ?
2.4.99.18 dolichyl diphosphooligosaccharide + synthetic tripeptides peptides that serve as acceptors show a secondary structural motif that involves the interaction between the asparagine side-chain carboxamide and the backbone amide of the threonine Sus scrofa ?
-
 ?