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Literature summary extracted from

  • Kamsteeg, J.; van Brederode, J.; van Nigtevecht, G.
    Identification and properties of UDP-glucose: cyanidin-3-O-glucosyltransferase isolated from petals of the red campion (Silene dioica) (1978), Biochem. Genet., 16, 1045-1058.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.115 2-mercaptoethanol detergent required for maximal activity Silene dioica
2.4.1.115 2-Methoxyethanol activation Silene dioica
2.4.1.115 cetrimide activation Silene dioica
2.4.1.115 cysteine detergent required for maximal activity Silene dioica
2.4.1.115 Triton X-100 activation Silene dioica
2.4.1.115 Tween 20 detergent required for maximal activity Silene dioica
2.4.1.115 Tween 80 detergent required for maximal activity Silene dioica

General Stability

EC Number General Stability Organism
2.4.1.115 repeated freeze-thawing inactivates Silene dioica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.115 CaCl2 2 mM, 18% inhibition Silene dioica
2.4.1.115 HgCl2 0.1 mM, 85% inhibition Silene dioica
2.4.1.115 NEM 1.25 mM, 30% inhibition Silene dioica
2.4.1.115 PCMB 1.25 mM, 93% inhhibition, 2-mercaptoethanol or cysteine restores activity Silene dioica
2.4.1.115 ZnCl2 2 mM, 22% inhibition Silene dioica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.115 0.07
-
 cyanidin
-
 Silene dioica
2.4.1.115 0.13
-
 delphinidin
-
 Silene dioica
2.4.1.115 0.13
-
 pelargonidin
-
 Silene dioica
2.4.1.115 0.41
-
 UDPglucose
-
 Silene dioica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.115 60000
-
 1 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
2.4.1.115 60000
-
 2 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
2.4.1.115 125000
-
 gel filtration Silene dioica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.115 UDPglucose + anthocyanidin Silene dioica involved in anthocyanine biosynthesis UDP + anthocyanidin-3-O-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.115 Silene dioica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.115 partial Silene dioica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.115 calyx
-
 Silene dioica
-
2.4.1.115 leaf young rosette leaves Silene dioica
-
2.4.1.115 petal highest activity in petals of opening flowers of young plants Silene dioica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.115 0.0252
-
-
 Silene dioica

Storage Stability

EC Number Storage Stability Organism
2.4.1.115 -17°C, about 40% loss of activity within 3 months Silene dioica
2.4.1.115 4°C, about 40% loss of activity within 1 week Silene dioica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.115 UDP-glucose + delphinidin more slowly than cyanidin Silene dioica UDP + delphinidin 3-O-glucoside
-
 ?
2.4.1.115 UDP-glucose + pelargonidin more slowly than cyanidin Silene dioica UDP + pelargonidin 3-O-glucoside
-
 ?
2.4.1.115 UDPglucose + anthocyanidin involved in anthocyanine biosynthesis Silene dioica UDP + anthocyanidin-3-O-glucoside
-
 ?
2.4.1.115 UDPglucose + cyanidin best substrate Silene dioica UDP + cyanidin 3-O-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.115 dimer 2 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica
2.4.1.115 monomer 1 * 60000, can exist as monomer or dimer, SDS-PAGE Silene dioica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.115 7.5
-
-
 Silene dioica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.115 6.6 8 50% of maximal activity at pH 6.6 and pH 8.0 Silene dioica