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Literature summary extracted from

  • Gruyer, S.; Legin, E.; Bliard, C.; Ball, S.; Duchiron, F.
    The endopolysaccharide metabolism of the hyperthermophilic archeon Thermococcus hydrothermalis: polymer structure and biosynthesis (2002), Curr. Microbiol., 44, 206-211.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.113 0.9
-
 ADP-glucose
-
 Thermococcus hydrothermalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.113 42000
-
 2 * 42000, SDS-PAGE Thermococcus hydrothermalis
2.4.1.113 85000
-
 gel filtration Thermococcus hydrothermalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.113 ADP-glucose + protein Thermococcus hydrothermalis
-
 ADP + alpha-D-glucosyl-protein
-
 ?
2.4.1.113 ADP-glucose + protein Thermococcus hydrothermalis AL662
-
 ADP + alpha-D-glucosyl-protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.113 Thermococcus hydrothermalis
-
 AL662
-
2.4.1.113 Thermococcus hydrothermalis AL662
-
 AL662
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.113 partial Thermococcus hydrothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.113 ADP-glucose + protein
-
 Thermococcus hydrothermalis ADP + alpha-D-glucosyl-protein
-
 ?
2.4.1.113 ADP-glucose + protein best donor substrate, enzyme can also use UDP-glucose as donor substrate Thermococcus hydrothermalis ADP + alpha-D-glucosyl-protein
-
 ?
2.4.1.113 ADP-glucose + protein
-
 Thermococcus hydrothermalis AL662 ADP + alpha-D-glucosyl-protein
-
 ?
2.4.1.113 ADP-glucose + protein best donor substrate, enzyme can also use UDP-glucose as donor substrate Thermococcus hydrothermalis AL662 ADP + alpha-D-glucosyl-protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.113 dimer 2 * 42000, SDS-PAGE Thermococcus hydrothermalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.113 80
-
-
 Thermococcus hydrothermalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.113 additional information
-
 no activity at 25°C and 110°C Thermococcus hydrothermalis
2.4.1.113 70 90 55% activity at 70°C and 90°C Thermococcus hydrothermalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.113 80
-
 stable for at least 2 h Thermococcus hydrothermalis
2.4.1.113 90
-
 half-life: 26 min, and 52 min in presence of 3.2 mM ADP-glucose Thermococcus hydrothermalis
2.4.1.113 100
-
 half-life: 3 min, and 9.5 min in presence of 3.2 mM ADP-glucose Thermococcus hydrothermalis
2.4.1.113 110
-
 inactivated, but in presence of 3.2 mM ADP-glucose the enzyme shows a half-life of 6 min Thermococcus hydrothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.113 5.5
-
-
 Thermococcus hydrothermalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.113 5.2 7.5 half-maximal activity at pH 5.2 and pH 7.5 Thermococcus hydrothermalis