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Literature summary extracted from

  • Fritz, T.A.; Gabb, M.M.; Wei, G.; Esko, J.D.
    Two N-acetylglucosaminyltransferases catalyze the biosynthesis of heparan sulfate (1994), J. Biol. Chem., 269, 28809-28814.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.101 CHAPS slight activation Cricetulus griseus
2.4.1.101 Triton X-100 slight activation Cricetulus griseus
2.4.1.101 Zwittergent 3-12 slight activation Cricetulus griseus
2.4.1.143 CHAPS
-
 Oncorhynchus mykiss
2.4.1.143 Triton X-100
-
 Oncorhynchus mykiss
2.4.1.143 Zwittergent 3-12
-
 Oncorhynchus mykiss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.101 0.036
-
 UDP-N-acetyl-D-glucosamine
-
 Cricetulus griseus
2.4.1.143 0.23
-
 UDP-N-acetylglucosamine pH 6.5, 37°C Oncorhynchus mykiss

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.101 microsome
-
 Cricetulus griseus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.101 Ca2+ can substitute Mn2+ Cricetulus griseus
2.4.1.101 Mn2+ requirement Cricetulus griseus
2.4.1.101 Mn2+ can also use Ca2+ Cricetulus griseus
2.4.1.143 Mn2+
-
 Oncorhynchus mykiss
2.4.1.143 additional information Ca2+, Co2+, Cu2+ and Mg2+ cannot replace Mn2+ Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.101 additional information Cricetulus griseus enzyme initiates the biosynthesis of heparan sulfate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Cricetulus griseus
-
 wild-type and mutant cell lines
-
2.4.1.143 Oncorhynchus mykiss
-
 CHO-cells
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.101 CHO cell wild-type and mutant F6 and F8 Cricetulus griseus
-
2.4.1.143 ovary
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.101 0.0000002
-
 wild-type S49 cell Cricetulus griseus
2.4.1.101 0.00000084
-
 wild-type CHO cell Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 additional information enzyme initiates the biosynthesis of heparan sulfate Cricetulus griseus ?
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Cricetulus griseus UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.101 UDP-N-acetyl-D-glucosamine + GlcUAcbeta1-3Galbeta1-O-naphthalenemethanol synthetic substrate Cricetulus griseus ?
-
 ?
2.4.1.143 UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
 Oncorhynchus mykiss UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetylglucosaminyl-R
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GlcNAc-T I
-
 Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 5 8 broad optimum Cricetulus griseus
2.4.1.143 5 8
-
 Oncorhynchus mykiss