EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.10 | Sodium dodecyl sulfate | slight activation at 5% w/v | Gluconacetobacter diazotrophicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.10 | expression in Acetobacter azotrophicus wild-type strain SRT4, 6fold increase in periplasm, 1.7fold increase of extracellular enzyme | Gluconacetobacter diazotrophicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.10 | 2-mercaptoethanol | slight inhibition at 50 mM | Gluconacetobacter diazotrophicus | |
2.4.1.10 | dithiothreitol | weak inhibition at 50 mM | Gluconacetobacter diazotrophicus | |
2.4.1.10 | proteinase K | 90% remaining activity after incubation at 37°C for 1 h | Gluconacetobacter diazotrophicus | |
2.4.1.10 | Urea | weak inhibition at 5 M | Gluconacetobacter diazotrophicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.10 | extracellular | signal peptide dependent secretion mechanism | Gluconacetobacter diazotrophicus | - |
- |
2.4.1.10 | periplasm | precursor form, before secretion into the medium, maturation includes cleavage of 30 amino acid residue signal peptide and formation of a pyroglutamic acid residue, not attached to membranes | Gluconacetobacter diazotrophicus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 60000 | - |
x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.10 | sucrose + (2,6-beta-D-fructosyl)n | Gluconacetobacter diazotrophicus | translocation of the enzyme is a rate-limiting step | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.10 | Gluconacetobacter diazotrophicus | - |
i.e. Acetobacter diazotrophicus | - |
2.4.1.10 | Gluconacetobacter diazotrophicus | - |
constitutive LsdA, strain SRT4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.10 | extracellular and periplasmic forms | Gluconacetobacter diazotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 2561 | - |
purified periplasmic enzyme | Gluconacetobacter diazotrophicus |
2.4.1.10 | 2603 | - |
purified extracellular enzyme | Gluconacetobacter diazotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.10 | sucrose + (2,6-beta-D-fructosyl)n | - |
Gluconacetobacter diazotrophicus | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
2.4.1.10 | sucrose + (2,6-beta-D-fructosyl)n | translocation of the enzyme is a rate-limiting step | Gluconacetobacter diazotrophicus | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.10 | ? | x * 60000, SDS-PAGE | Gluconacetobacter diazotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 40 | - |
assay at | Gluconacetobacter diazotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.10 | 5.2 | - |
assay at | Gluconacetobacter diazotrophicus |