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Literature summary extracted from

  • Preiss, J.; Okita, T.W.; Greenberg, E.
    Characterization of the spinach leaf phosphorylases (1980), Plant Physiol., 66, 864-869.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 0.9
-
 phosphate cytoplasmic phosphorylase, pH 7.0 Spinacia oleracea
2.4.1.1 1.2
-
 phosphate chloroplast phosphorylase, pH 7.0 Spinacia oleracea
2.4.1.1 1.5
-
 phosphate cytoplasmic phosphorylase, pH 8.0 Spinacia oleracea
2.4.1.1 1.8
-
 phosphate chloroplastphosphorylase, pH 8.0 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 chloroplast
-
 Spinacia oleracea 9507
-
2.4.1.1 cytoplasm
-
 Spinacia oleracea 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 92000
-
 2 * 92000, cytoplasmic isozyme, SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 cytoplasmic phosphorylase: ammonium sulfate, DEAE-cellulose, glycogen-Sepharose, chloroplast phosphorylase: DEAE-cellulose, Biogel A, partial purification Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 0.16
-
 chloroplastic enzyme, phosphorolysis Spinacia oleracea
2.4.1.1 43.5
-
 cytoplasmic enzyme, synthesis Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + glucose 1-phosphate effective primer Spinacia oleracea amylopectin + phosphate
-
 r
2.4.1.1 amylose + glucose 1-phosphate effective primer Spinacia oleracea amylose + phosphate
-
 r
2.4.1.1 amylose + glucose 1-phosphate potato amylose Spinacia oleracea amylose + phosphate
-
 r
2.4.1.1 debranched amylopectin + glucose 1-phosphate most effective primer glucan for chloroplastic enzyme Spinacia oleracea debranched amylopectin + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from: rabbit liver Spinacia oleracea glycogen + phosphate
-
 r
2.4.1.1 maltopentaose + glucose 1-phosphate cytoplasmic enzyme, glucosylation at the same rate of glucosylation of debranched amylopectin, chloroplastic enzyme, glucosylation at 50% the rate of glucosylation of debranched amylopectin Spinacia oleracea maltohexaose + phosphate
-
 r
2.4.1.1 maltotetraose + glucose 1-phosphate cytoplasmic enzyme: poor substrate Spinacia oleracea maltopentaose + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Spinacia oleracea [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 dimer 2 * 92000, cytoplasmic isozyme, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6 7.5 cytoplasmic enzyme, buffer dependent optima in this range Spinacia oleracea
2.4.1.1 7.5
-
 broad optimum, chloroplast enzyme, phosphorolysis Spinacia oleracea