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Literature summary extracted from

  • Kumar, A.; Sanwal, G.G.
    Purification and physicochemical properties of starch phosphorylase from young banana leaves (1982), Biochemistry, 21, 4152-4159.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 fumarate activation Musa x paradisiaca
2.4.1.1 Maleic acid activation Musa x paradisiaca
2.4.1.1 additional information not activated by indoleacetic acid and gibberellic acid Musa x paradisiaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 adenine
-
 Musa x paradisiaca
2.4.1.1 Ag+ 2 mM, 78% inhibition Musa x paradisiaca
2.4.1.1 Antiserum of purified phosphorylase
-
 Musa x paradisiaca
2.4.1.1 aromatic amino acid
-
 Musa x paradisiaca
2.4.1.1 ATP 1 mM, 50% inhibition, at non-saturating levels of glucose 1-phosphate; additive inhibition together with tyrosine Musa x paradisiaca
2.4.1.1 Cu2+ 2 mM, 73% inhibition Musa x paradisiaca
2.4.1.1 Fe2+ 2 mM, 73% inhibition Musa x paradisiaca
2.4.1.1 guanine
-
 Musa x paradisiaca
2.4.1.1 Hg2+ 2 mM, complete inhibition Musa x paradisiaca
2.4.1.1 L-tyrosine additive inhibition together with ATP Musa x paradisiaca
2.4.1.1 monohydroxyphenols 10 mM, no inhibition at 1 mM Musa x paradisiaca
2.4.1.1 additional information not inhibited by 3-phosphoglycerate; not inhibited by adenosine, cytosine; not inhibited by F-, ClO4-; not inhibited by fructose 6-phosphate; not inhibited by GMP; not inhibited by non-aromatic amino acids; not inhibited by succinate, 2-oxoglutarate, malate Musa x paradisiaca
2.4.1.1 p-chloromercuribenzoate 0.5 mM, 60% inhibition, reversible by 10 mM 2-mercaptoethanol Musa x paradisiaca
2.4.1.1 UDPglucose 10 mM, 44% inhibition at non-saturating levels of glucose 1-phosphate Musa x paradisiaca
2.4.1.1 Zn2+ 2 mM, complete inhibition Musa x paradisiaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 0.13 mg/ml, starch phosphorolysis Musa x paradisiaca
2.4.1.1 additional information
-
 additional information 0.19 mg/ml, starch synthesis Musa x paradisiaca
2.4.1.1 0.66
-
 glucose 1-phosphate starch synthesis Musa x paradisiaca
2.4.1.1 10.87
-
 phosphate starch phosphorolysis Musa x paradisiaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 additional information
-
 Musa x paradisiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 55000
-
 8 * 55000, SDS-PAGE Musa x paradisiaca

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Musa x paradisiaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 ammonium sulfate, DEAE-cellulose, Sephadex G100, Sephadex g-200, starch-Sepharose affinity chromatography Musa x paradisiaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 leaf
-
 Musa x paradisiaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 1.5
-
-
 Musa x paradisiaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate less effective than starch, amylose or glycogen Musa x paradisiaca (dextrin)n + phosphate
-
 r
2.4.1.1 amylopectin + glucose 1-phosphate less effective than starch, amylose or glycogen Musa x paradisiaca amylopectin + phosphate
-
 r
2.4.1.1 amylose + glucose 1-phosphate best substrate Musa x paradisiaca amylose + phosphate
-
 ?
2.4.1.1 glycogen + glucose 1-phosphate best substrate Musa x paradisiaca glycogen + phosphate
-
 r
2.4.1.1 maltose + alpha-D-glucose 1-phosphate less effective than starch, amylose or glycogen Musa x paradisiaca maltotriose + phosphate
-
 ?
2.4.1.1 maltotriose + glucose 1-phosphate less effective than starch, amylose or glycogen Musa x paradisiaca maltotetraose + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate best substrate Musa x paradisiaca starch + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate soluble starch Musa x paradisiaca starch + phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with sucrose Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with ribose 5-phosphate Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate strict specificity for glucose 1-phosphate Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate no activity with Schardinger dextrin and cellulose Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate polyglucose primer required Musa x paradisiaca [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.1 octamer 8 * 55000, SDS-PAGE Musa x paradisiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 40
-
-
 Musa x paradisiaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 additional information
-
 heating enhances sensitivity to tyrosine inhibition Musa x paradisiaca
2.4.1.1 55
-
 about 40% loss of activity within 5 min Musa x paradisiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6
-
 synthesis Musa x paradisiaca
2.4.1.1 7.5
-
-
 Musa x paradisiaca

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.1 additional information absence of pyridoxal 5'-phosphate Musa x paradisiaca