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Literature summary extracted from

  • Robson, R.L.; Morris, J.G.
    Mobilization of granulose in Clostridium pasteurianum. Purification and properties of granulose phosphorylase (1974), Biochem. J., 144, 513-517.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 5'-AMP activation Clostridium pasteurianum
2.4.1.1 5'-AMP slight activation Clostridium pasteurianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 ADPglucose
-
 Clostridium pasteurianum
2.4.1.1 ADPmannose
-
 Clostridium pasteurianum
2.4.1.1 ADPribose
-
 Clostridium pasteurianum
2.4.1.1 ATP less effective than nucleotide sugars Clostridium pasteurianum
2.4.1.1 dTDPglucose
-
 Clostridium pasteurianum
2.4.1.1 GDPglucose
-
 Clostridium pasteurianum
2.4.1.1 glucose 1-phosphate not inhibited Clostridium pasteurianum
2.4.1.1 additional information not inhibited by 3-phosphoglycerate; not inhibited by acetate, acetylphosphate, butyrylphosphate, 6-phosphogluconate; not inhibited by fructose 6-phosphate Clostridium pasteurianum
2.4.1.1 NADPH also oxidized form, less effective than nucleotide sugars, not NAD(H) Clostridium pasteurianum
2.4.1.1 UDPglucose
-
 Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information
-
 Clostridium pasteurianum
2.4.1.1 0.05
-
 glucose 1-phosphate glucan synthesis Clostridium pasteurianum
2.4.1.1 0.55
-
 glucose 1-phosphate glucan synthesis Clostridium pasteurianum
2.4.1.1 2.2
-
 phosphate phosphorolysis of glucan Clostridium pasteurianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.1 cytoplasm loosely bound to native granules of its substrate polyglucan Clostridium pasteurianum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.1 additional information no divalent cations required Clostridium pasteurianum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Clostridium pasteurianum
-
 ATCC 6013
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 partial Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 1.9
-
-
 Clostridium pasteurianum

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 0°C, several days Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + glucose 1-phosphate
-
 Clostridium pasteurianum amylopectin + phosphate
-
 r
2.4.1.1 granulose + glucose 1-phosphate
-
 Clostridium pasteurianum granulose + phosphate
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Clostridium pasteurianum [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.4
-
-
 Clostridium pasteurianum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.02
-
 ADP-glucose
-
 Clostridium pasteurianum
2.4.1.1 0.02
-
 GDP-glucose
-
 Clostridium pasteurianum
2.4.1.1 0.06
-
 UDP-glucose
-
 Clostridium pasteurianum
2.4.1.1 2.74
-
 NADPH
-
 Clostridium pasteurianum