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Literature summary extracted from

  • Khandelwal, R.L.; Spearman, T.N.; Hamilton, I.R.
    Purification and properties of glycogen phosphorylase from Streptococcus salivarius (1973), Arch. Biochem. Biophys., 154, 295-305.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 2-mercaptoethanol activation, 5 mM Streptococcus salivarius
2.4.1.1 cysteine activation Streptococcus salivarius
2.4.1.1 Mercaptoacetic acid 1 mM, activation, inhibition at 5 mM Streptococcus salivarius
2.4.1.1 p-hydroxymercuribenzoate slight activation, 0.1-1.0 mM Streptococcus salivarius

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 Ca2+ weak Streptococcus salivarius
2.4.1.1 Co2+
-
 Streptococcus salivarius
2.4.1.1 Cu2+ 2 mM, 98% inhibition Streptococcus salivarius
2.4.1.1 DTNB 1 mM, weak Streptococcus salivarius
2.4.1.1 Fe2+ 2 mM, 60% inhibition in the presence of 1 mM AMP Streptococcus salivarius
2.4.1.1 Mg2+ slight Streptococcus salivarius
2.4.1.1 Mn2+
-
 Streptococcus salivarius
2.4.1.1 additional information not inhibited by 2-mercaptoethanol; not inhibited by Sr2+ Streptococcus salivarius
2.4.1.1 Sn2+
-
 Streptococcus salivarius
2.4.1.1 Zn2+ 2 mM, 63% inhibition in the presence of 1 mM AMP Streptococcus salivarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.1 additional information
-
 additional information 20 mg/ml, oyster glycogen, phosphorolysis, 25 mg/ml, endogen glycogen, synthesis, 27 mg/ml, corn dextrin, phosphorolysis, 40 mg/ml, corn dextrin, synthesis Streptococcus salivarius
2.4.1.1 additional information
-
 additional information 42 mg/ml, oyster glycogen, synthesis Streptococcus salivarius
2.4.1.1 3.4
-
 glucose 1-phosphate glycogen synthesis Streptococcus salivarius
2.4.1.1 3.4
-
 Glucose-1-phosphate glycogen synthesis Streptococcus salivarius
2.4.1.1 20
-
 phosphate phosphorolysis of oyster glycogen Streptococcus salivarius
2.4.1.1 25
-
 phosphate phosphorolysis of corn dextrin Streptococcus salivarius

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Streptococcus salivarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 protamine sulfate, ammonium sulfate, DEAE-Sephadex, Sephadex G-200 Streptococcus salivarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 0.005
-
-
 Streptococcus salivarius

Storage Stability

EC Number Storage Stability Organism
2.4.1.1 -10°C, 200 mM NaF, 1 month, no loss of activity Streptococcus salivarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate best substrate Streptococcus salivarius (dextrin)n + phosphate
-
 r
2.4.1.1 (dextrin)n-1 + glucose 1-phosphate corn dextrin Streptococcus salivarius (dextrin)n + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate about half as effective as dextrin Streptococcus salivarius glycogen + phosphate
-
 r
2.4.1.1 glycogen + glucose 1-phosphate glycogen from oyster Streptococcus salivarius glycogen + phosphate
-
 r
2.4.1.1 starch + glucose 1-phosphate glucosylation at 32% the rate of dextrin glucosylation Streptococcus salivarius starch + phosphate
-
 ?
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Streptococcus salivarius [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.1 37
-
 2 h stable Streptococcus salivarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6
-
-
 Streptococcus salivarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.1 5.7 7.2 approx. half-maximal activity at pH 5.7 and 7.2 Streptococcus salivarius