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Literature summary extracted from

  • Goschalk, G.; Dittbrenner, S.
    Properties of (R)-citrate synthase from Clostridium acici-urici (1970), Hoppe-Seyler's Z. Physiol. chem., 351, 1183-1190.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.3 2-mercaptoethanol purified enzyme does not require 2-mecaptoethanol or anaerobic conditiones for maximal activity Clostridium acidi-urici

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.3 2-oxoglutarate 5 mM, 64% inhibition, competitive vs. oxaloacetate Clostridium acidi-urici
2.3.3.3 4-chloromercuribenzoate strong inhibition Clostridium acidi-urici
2.3.3.3 Cd2+ complete inactivation Clostridium acidi-urici
2.3.3.3 H2O2
-
 Clostridium acidi-urici
2.3.3.3 Hg2+ complete inactivation Clostridium acidi-urici
2.3.3.3 iodoacetamide strong inhibition Clostridium acidi-urici
2.3.3.3 additional information not inhibited by 5,5'-dithiobis(2-nitrobenzoate) Clostridium acidi-urici
2.3.3.3 Zn2+ above 0.02 mM, activation below Clostridium acidi-urici

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.3 0.04
-
 oxaloacetate
-
 Clostridium acidi-urici
2.3.3.3 0.05
-
 acetyl-CoA
-
 Clostridium acidi-urici

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.3 Co2+ can replace Mn2+ Clostridium acidi-urici
2.3.3.3 Zn2+ activation below 0.02 mM, inhibition above Clostridium acidi-urici

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.3 Clostridium acidi-urici
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.3 isoelectric precipitate, DEAE-cellulose, ammonium sulfate, DEAE-Sephadex, ammonium sulfate, gel filtration Clostridium acidi-urici

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.3 5.5
-
-
 Clostridium acidi-urici

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate no activity with propionyl-CoA, glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoglutarate, 2-oxoisovalerate and 2-oxoisocaproate Clostridium acidi-urici citrate + CoA
-
 ?