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Literature summary extracted from

  • Wulandari, A.P.; Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Hoshino, T.; Yamane, H.
    Characterization of bacterial homocitrate synthase involved in lysine biosynthesis (2002), FEBS Lett., 522, 35-40.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.14 acetyl-CoA
-
 Thermus thermophilus
2.3.3.14 L-Lys
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.14 0.028
-
 acetyl-CoA
-
 Thermus thermophilus
2.3.3.14 0.032
-
 acetyl-CoA
-
 Thermus thermophilus
2.3.3.14 0.044
-
 2-oxoglutarate
-
 Thermus thermophilus
2.3.3.14 0.255
-
 oxaloacetate
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.14 60000
-
 gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate Thermus thermophilus first enzyme of the lysine biosynthetic pathway 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.14
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Thermus thermophilus 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate first enzyme of the lysine biosynthetic pathway Thermus thermophilus 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
2.3.3.14 acetyl-CoA + H2O + oxaloacetate
-
 Thermus thermophilus 2-hydroxypropane-1,2,3-tricarboxylate + CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.14 60
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.14 40 80 40°C: about 70% of maximal activity, 80°C: about 60% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.14 70
-
 rapid loss of activity above Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.14 0.967
-
 acetyl-CoA
-
 Thermus thermophilus
2.3.3.14 0.967
-
 oxaloacetate
-
 Thermus thermophilus
2.3.3.14 1.53
-
 acetyl-CoA reaction with 2-oxoglutarate Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.14 0.0094
-
 L-Lys
-
 Thermus thermophilus