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Literature summary extracted from

  • Zheng, L.; White, H.R.; Dean, D.R.
    Purification of the Acobacter vinelandii nifV-encoded homocitrate synthase (1997), J. Bacteriol., 179, 5963-5966.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.14 expressed at high levels in Escherichia coli Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.14 0.06
-
 acetyl-CoA reaction with 2-oxoglutarate Azotobacter vinelandii
2.3.3.14 1.24
-
 2-oxoadipate
-
 Azotobacter vinelandii
2.3.3.14 2.24
-
 2-oxoglutarate
-
 Azotobacter vinelandii
2.3.3.14 2.28
-
 oxaloacetate
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.14 89000
-
 gel filtration Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.14 Azotobacter vinelandii
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.3.14 loss of approximately 50% of its activity within 2 h upon exposure to air Azotobacter vinelandii

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.14 recombinant enzyme Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.14 0.657
-
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.14 acetyl-CoA + H2O + 2-oxoadipate
-
 Azotobacter vinelandii 2-hydroxyhexane-1,2,6-tricarboxylate
-
 ?
2.3.3.14 acetyl-CoA + H2O + 2-oxoglutarate
-
 Azotobacter vinelandii 2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?
2.3.3.14 acetyl-CoA + H2O + oxaloacetate
-
 Azotobacter vinelandii 2-hydroxypropane-1,2,3-tricarboxylate + CoA
-
 ?