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Literature summary extracted from

  • Chun, K.Y.; Vinarov, D.A.; Zajicek, J.; Miziorko, H.M.
    3-hydroxy-3-methylglutaryl-CoA synthase. A role for glutamate 95 in general acid/base catalysis of C-C bond formation (2000), J. Biol. Chem., 275, 17946-17953.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.10 medicine target of a potent antisteroidogenic drug Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10
-
 Homo sapiens
2.3.3.10 expression vector pET-3d, cloned and expressed in Escherichia coli BL21 (DE3) Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 3-carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxyl-CoA
-
 Gallus gallus
2.3.3.10 3-chloropropionyl-CoA
-
 Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.0012
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.011
-
 acetyl-CoA acetyl-CoA hydrolysis, wild-type Gallus gallus
2.3.3.10 0.012
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 0.014
-
 acetyl-CoA acetyl-CoA hydrolysis, mutant E95A Gallus gallus
2.3.3.10 0.29
-
 acetyl-CoA wild-type enzyme Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 57600
-
 2 * 57600 Gallus gallus
2.3.3.10 116000
-
 gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Methanothermobacter thermautotrophicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Arabidopsis thaliana
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Blattella germanica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Borreliella burgdorferi
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Mus musculus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Pinus sylvestris
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Schizosaccharomyces pombe
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Sus scrofa
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Arabidopsis thaliana P54873
-
-
2.3.3.10 Blattella germanica P54870 cockroach, cytosolic isoform 2
-
2.3.3.10 Blattella germanica P54961 cockroach, cytosolic isoform 1
-
2.3.3.10 Borreliella burgdorferi O51626
-
-
2.3.3.10 Gallus gallus
-
 avian
-
2.3.3.10 Gallus gallus P23228 chicken, cytosolic isoform
-
2.3.3.10 Homo sapiens
-
 human
-
2.3.3.10 Homo sapiens P54868 human, mitochondrial isoform
-
2.3.3.10 Methanothermobacter thermautotrophicus
-
-
-
2.3.3.10 Mus musculus P54869 mouse, mitochondrial isoform
-
2.3.3.10 Pinus sylvestris P93773 pine
-
2.3.3.10 Rattus norvegicus P22791 rat, mitochondrial isoform
-
2.3.3.10 Schizosaccharomyces pombe P54874
-
-
2.3.3.10 Sus scrofa O02734 pig, mitochondrial isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Methanothermobacter thermautotrophicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Arabidopsis thaliana (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Blattella germanica (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Borreliella burgdorferi (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Mus musculus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Pinus sylvestris (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Schizosaccharomyces pombe (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Sus scrofa (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer
-
 Homo sapiens
2.3.3.10 dimer 2 * 57600 Gallus gallus