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Literature summary extracted from

  • Rokosz, L.L.; Boulton, D.A.; Butkiewicz, E.A.; Sanyal, G.; Cueto, M.A.; Lachance, P.A.; Hermes, J.D.
    Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: expression, purification, andcharacterization of recombinant wild-type and cys-129 mutant enzymes (1994), Arch. Biochem. Biophys., 312, 1-13.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.10 medicine deficiency of the mitochondrial isoform correlates with human metabolic disease, cytosolic isoform is potentially useful target for drugs aimed at lowering cholesterol levels Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 cDNA subcloned and expressed from a T7-based vector in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 acetoacetyl-CoA
-
 Gallus gallus
2.3.3.10 acetoacetyl-CoA
-
 Homo sapiens
2.3.3.10 acetoacetyl-CoA
-
 Rattus norvegicus
2.3.3.10 L-659,699 potent inhibitor of the recombinant enzyme Homo sapiens
2.3.3.10 L-659,699
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.029
-
 acetyl-CoA
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.10 cytoplasm
-
 Gallus gallus 5737
-
2.3.3.10 cytoplasm
-
 Homo sapiens 5737
-
2.3.3.10 cytoplasm
-
 Rattus norvegicus 5737
-
2.3.3.10 cytosol
-
 Gallus gallus 5829
-
2.3.3.10 cytosol
-
 Homo sapiens 5829
-
2.3.3.10 cytosol
-
 Rattus norvegicus 5829
-
2.3.3.10 mitochondrion
-
 Gallus gallus 5739
-
2.3.3.10 mitochondrion
-
 Homo sapiens 5739
-
2.3.3.10 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 120000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Gallus gallus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Cricetulus griseus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Rattus norvegicus
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Cricetulus griseus
-
 chinese hamster
-
2.3.3.10 Gallus gallus
-
 chicken
-
2.3.3.10 Homo sapiens
-
 human
-
2.3.3.10 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.10 adrenal gland
-
 Homo sapiens
-
2.3.3.10 adrenal gland
-
 Rattus norvegicus
-
2.3.3.10 fetus
-
 Homo sapiens
-
2.3.3.10 fibroblast
-
 Homo sapiens
-
2.3.3.10 liver
-
 Gallus gallus
-
2.3.3.10 liver
-
 Rattus norvegicus
-
2.3.3.10 ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 0.76
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Gallus gallus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Cricetulus griseus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Rattus norvegicus (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.10 dimer
-
 Gallus gallus
2.3.3.10 dimer
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.0000537
-
 L-659,699
-
 Homo sapiens
2.3.3.10 0.012
-
 acetoacetyl-CoA
-
 Homo sapiens