Literature summary extracted from

Allred, J.B.
Properties and subcellular distribution of enzymes required for acetoacetate biosynthesis in chicken liver (1973), Biochim. Biophys. Acta, 297, 22-30.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.3.10	Cu2+	-	Gallus gallus
2.3.3.10	Zn2+	-	Gallus gallus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.3.3.10	cytoplasm	-	Gallus gallus	5737	-
2.3.3.10	cytoplasm	-	Cavia porcellus	5737	-
2.3.3.10	cytoplasm	-	Rattus norvegicus	5737	-
2.3.3.10	mitochondrion	-	Gallus gallus	5739	-
2.3.3.10	mitochondrion	-	Cavia porcellus	5739	-
2.3.3.10	mitochondrion	-	Rattus norvegicus	5739	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.3.10	Mn2+	increases acetoacetate synthesis activity	Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	Cavia porcellus	-	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	Rattus norvegicus	-	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	Gallus gallus	acetoacetate biosynthesis, 3-hydroxy-3-methylglutaryl-CoA pathway	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.3.10	Cavia porcellus	-	guinea pig	-
2.3.3.10	Gallus gallus	-	chicken	-
2.3.3.10	Rattus norvegicus	-	rat	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.3.3.10	liver	-	Gallus gallus	-
2.3.3.10	liver	-	Cavia porcellus	-
2.3.3.10	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.3.10	0.00083	-	mitochondrial pellet	Gallus gallus
2.3.3.10	0.00133	-	mitochondrial pellet, 0.25 M sucrose	Gallus gallus
2.3.3.10	0.00245	-	disrupted mitochondria, 0.25 M sucrose	Gallus gallus
2.3.3.10	0.00288	-	mitochondrial pellet, 0.50 M sucrose	Gallus gallus
2.3.3.10	0.00325	-	mitochondrial pellet, 0.88 M sucrose	Gallus gallus
2.3.3.10	0.00485	-	homogenate	Gallus gallus
2.3.3.10	0.00567	-	disrupted mitochondria, 0.50 M sucrose	Gallus gallus
2.3.3.10	0.00792	-	disrupted mitochondria, 0.88 M sucrose	Gallus gallus
2.3.3.10	0.00922	-	mitochondrial supernatant	Gallus gallus
2.3.3.10	0.00958	-	mitochondrial supernatant, 0.88 M sucrose	Gallus gallus
2.3.3.10	0.01053	-	mitochondrial supernatant, 0.50 M sucrose	Gallus gallus
2.3.3.10	0.0111	-	mitochondrial supernatant, 0.25 M sucrose	Gallus gallus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	-	Gallus gallus	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	-	Cavia porcellus	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	-	Rattus norvegicus	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?
2.3.3.10	acetyl-CoA + acetoacetyl-CoA + H2O	acetoacetate biosynthesis, 3-hydroxy-3-methylglutaryl-CoA pathway	Gallus gallus	(S)-3-hydroxy-3-methylglutaryl-CoA + CoA	-	?

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Release 2023.1 (January 2023)