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Literature summary extracted from

  • Middleton, B.
    The kinetic mechanism of 3-hydroxy-3-methylglutaryl-coenzyme a synthase from baker's yeast (1972), Biochem. J., 126, 35-47.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 3,3-dimethylglutaryl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 3-oxohexanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 acetoacetyl-CoA substrate inhibition Saccharomyces cerevisiae
2.3.3.10 butyryl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 CoA
-
 Saccharomyces cerevisiae
2.3.3.10 decanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 desulfo-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 DL-3-hydroxy-3-methylglutaryl-CoA uncompetitive inhibition Saccharomyces cerevisiae
2.3.3.10 DL-3-methylglutaryl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 glutaryl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 heptanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 hexanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 nonanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 octanoyl-CoA
-
 Saccharomyces cerevisiae
2.3.3.10 propionyl-CoA
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.0004
-
 acetoacetyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.0032
-
 acetoacetyl-CoA pH 8.9 Saccharomyces cerevisiae
2.3.3.10 0.014
-
 acetyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.018
-
 acetyl-CoA pH 8.9 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O Saccharomyces cerevisiae synthesis of isoprenes and steroids (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.10 2
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O
-
 Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 acetyl-CoA + acetoacetyl-CoA + H2O synthesis of isoprenes and steroids Saccharomyces cerevisiae (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir
2.3.3.10 additional information acetyl-CoA homologues do not act as substrates Saccharomyces cerevisiae ?
-
 ?
2.3.3.10 additional information absolutely specific for acetyl-CoA and acetoacetyl-CoA in the reaction, neither propionyl- nor butyryl-CoA can replace acetyl-CoA, no reaction observed when 3-oxohexanoyl-CoA is substituted for acetoacetyl-CoA Saccharomyces cerevisiae ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.0027
-
 decanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.004
-
 nonanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.005
-
 propionyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.0071
-
 3-oxohexanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.008
-
 acetoacetyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.0081
-
 octanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.0123
-
 heptanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.013
-
 DL-3-hydroxy-3-methylglutaryl-CoA pH 8.0, competitive inhibition, substrate acetyl-CoA Saccharomyces cerevisiae
2.3.3.10 0.014
-
 3-oxohexanoyl-CoA pH 8.0, mixed inhibition, substrate acetoacetyl-CoA Saccharomyces cerevisiae
2.3.3.10 0.02
-
 acetoacetyl-CoA pH 8.9 Saccharomyces cerevisiae
2.3.3.10 0.022
-
 hexanoyl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.03
-
 desulfo-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.033
-
 butyryl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.038
-
 CoA pH 8.0, mixed inhibition, substrate acetyl-CoA Saccharomyces cerevisiae
2.3.3.10 0.06
-
 CoA pH 8.0, mixed inhibition, substrate acetoacetyl-CoA Saccharomyces cerevisiae
2.3.3.10 0.07
-
 desulfo-CoA pH 8.0, mixed inhibition, substrate acetoacetyl-CoA Saccharomyces cerevisiae
2.3.3.10 0.1
-
 DL-3-methylglutaryl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.11
-
 glutaryl-CoA pH 8.0 Saccharomyces cerevisiae
2.3.3.10 0.12
-
 3,3-dimethylglutaryl-CoA pH 8.0 Saccharomyces cerevisiae