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Literature summary extracted from

  • Kocabiyik, S.; Erduran, I.
    The effect of valine substitution for glycine in the dimer interface of citrate synthase from Thermoplasma acidophilum on stability and activity (2000), Biochem. Biophys. Res. Commun., 275, 460-465.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.16 Guanidinium chloride activation of recombinant wild-type 1.6fold at 0.5 M, inactivation at 1.6 M Thermoplasma acidophilum
2.3.3.16 Urea activation of recombinant wild-type by 35% at up to 8 M, inactivation at 9.3 M Thermoplasma acidophilum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.16 expression of wild-type and mutant in Escherichia coli Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 G196V site-directed mutagenesis, mutation interferes with dimerization, improper dimerization or dissociation of the dimer, reduced enzyme activity and conformational stability Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 Guanidinium chloride irreversible inactivation of recombinant wild-type at 1.6 M and of recombinant mutant G196V at 0.2 M, at 0.5 M activation of the wild-type Thermoplasma acidophilum
2.3.3.16 Urea irreversible inactivation of recombinant wild-type at 9.3 M and of recombinant mutant G196V at 5 M, at up to 8 M activation of the wild-type Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0045
-
acetyl-CoA recombinant mutant G196V Thermoplasma acidophilum
2.3.3.16 0.005
-
acetyl-CoA recombinant wild-type Thermoplasma acidophilum
2.3.3.16 0.0057
-
oxaloacetate recombinant mutant G196V Thermoplasma acidophilum
2.3.3.16 0.0066
-
oxaloacetate recombinant wild-type Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16 recombinant wild-type and mutant G196V from E. coli Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 0.008
-
recombinant purified mutant G196V Thermoplasma acidophilum
2.3.3.16 0.083
-
recombinant purified wild-type Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
Thermoplasma acidophilum citrate + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer
-
Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 CS
-
Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 55
-
enzyme assay at Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.16 80
-
t1/2 of recombinant wild-type is 10 min, t1/2 of recombinant mutant G196V is 8 min, inactivation of the mutant occurs in 2 stages: a first slow and a second rapid one Thermoplasma acidophilum
2.3.3.16 90
-
mutant G196V: t1/2: below 1 min, t1/2 wild-type: 5 min Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 0.0133
-
acetyl-CoA recombinant mutant G196V Thermoplasma acidophilum
2.3.3.16 0.15
-
acetyl-CoA recombinant wild-type Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8
-
enzyme assay at Thermoplasma acidophilum