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Literature summary extracted from

  • Belova, L.L.; Sokolov, A.P.; Morgunov, I.G.; Trotsenko, YuA.
    Purification and characterization of citrate synthase from Methylobacterium extorquens - a methylotrophic producer of polyhydroxybutyrate (1997), Biochemistry, 62, 71-76.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 2-oxoglutarate no inhibition Methylorubrum extorquens
2.3.3.16 ADP no inhibition Methylorubrum extorquens
2.3.3.16 ATP
-
 Methylorubrum extorquens
2.3.3.16 NADH no effect Methylorubrum extorquens
2.3.3.16 NADPH no inhibition Methylorubrum extorquens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.012
-
 oxaloacetate
-
 Methylorubrum extorquens
2.3.3.16 0.084
-
 acetyl-CoA
-
 Methylorubrum extorquens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl up to 80 mM Methylorubrum extorquens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 66000
-
 4 * 66000, SDS-PAGE Methylorubrum extorquens
2.3.3.16 260000
-
 gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Methylorubrum extorquens involved in poly 3-hydroxybutyrate synthesis citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Methylorubrum extorquens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Methylorubrum extorquens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 24
-
 purified enzyme Methylorubrum extorquens

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 4°C, Tris-HCl buffer, pH8.0, 50 mM glycerol, at least 1 week Methylorubrum extorquens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Methylorubrum extorquens citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O involved in poly 3-hydroxybutyrate synthesis Methylorubrum extorquens citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 tetramer 4 * 66000, SDS-PAGE Methylorubrum extorquens

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 CS
-
 Methylorubrum extorquens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 30
-
 enzyme assay at Methylorubrum extorquens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 8.6 9
-
 Methylorubrum extorquens