Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kojima, H.; Chiba, J.; Watanabe, Y.; Numata, O.
    Citrate synthase purified from Tetrahymena mitochondria is identical with Tetrahymena 14-nm filament protein (1995), J. Biochem., 118, 189-195.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ATP
-
 Tetrahymena pyriformis
2.3.3.16 additional information inhibited by a specific antibody against 49K protein Tetrahymena pyriformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0283
-
 oxaloacetate
-
 Tetrahymena pyriformis
2.3.3.16 0.0286
-
 acetyl-CoA
-
 Tetrahymena pyriformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.16 mitochondrion
-
 Tetrahymena pyriformis 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl maximum activity at 150 mM Tetrahymena pyriformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 49000
-
 x * 49000, SDS-PAGE Tetrahymena pyriformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Tetrahymena pyriformis also acting as structural protein in oral morphogenesis and conjugation citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O Tetrahymena pyriformis W also acting as structural protein in oral morphogenesis and conjugation citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Tetrahymena pyriformis
-
-
-
2.3.3.16 Tetrahymena pyriformis
-
 identical with 14-nm filament protein, i.e. 49K protein
-
2.3.3.16 Tetrahymena pyriformis W
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Tetrahymena pyriformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 35.1
-
 purified enzyme Tetrahymena pyriformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Tetrahymena pyriformis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O also acting as structural protein in oral morphogenesis and conjugation Tetrahymena pyriformis citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Tetrahymena pyriformis W citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + oxaloacetate + H2O also acting as structural protein in oral morphogenesis and conjugation Tetrahymena pyriformis W citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 ? x * 49000, SDS-PAGE Tetrahymena pyriformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 7.8
-
-
 Tetrahymena pyriformis