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Literature summary extracted from

  • Pereira, D.S.; Donald, L.J.; Hesfield, D.J.; Duckworth, H.W.
    Active site mutants of Escherichia coli citrate synthase (1994), J. Biol. Chem., 269, 412-417.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.16 D362A acetyl-CoA binding site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli
2.3.3.16 F383A acetyl-CoA binding site mutant, reduced turnover Escherichia coli
2.3.3.16 H229Q active site mutant, reduced turnover, increased Ki for 2-oxoglutarate Escherichia coli
2.3.3.16 H264A acetyl-CoA binding site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli
2.3.3.16 H305A active site mutant, reduced turnover Escherichia coli
2.3.3.16 additional information effect of active-site mutantions on substrate binding Escherichia coli
2.3.3.16 R314L active site mutant, reduced turnover Escherichia coli
2.3.3.16 R387L active site mutant, reduced turnover Escherichia coli
2.3.3.16 R407L active site mutant, reduced turnover, increased Ki for oxaloacetate and 2-oxoglutarate Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 2-oxoglutarate wild-type and mutants Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 additional information
-
 additional information mutants, with and without KCl, overview Escherichia coli
2.3.3.16 0.026
-
 oxaloacetate wild-type Escherichia coli
2.3.3.16 0.12
-
 acetyl-CoA wild-type Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl mutants, overview Escherichia coli
2.3.3.16 KCl 39fold activation at saturating concentration, wild-type Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Escherichia coli citrate + CoA
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 additional information
-
 additional information mutants, overview Escherichia coli
2.3.3.16 0.76
-
 2-oxoglutarate wild-type Escherichia coli