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Literature summary extracted from

  • Takahashi, R.; Usui, K.; Sakuraba, T.; Tokuyama, T.
    Purification and some properties of citrate synthase from a nitrite-oxidising chemoautotroph, Nitrobacter agilis ATCC 14123 (1994), J. Ferment. Bioeng., 77, 97-99.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.1 2-oxoglutarate no inhibition Nitrobacter winogradskyi
2.3.3.1 ATP no inhibition Nitrobacter winogradskyi
2.3.3.1 CuSO4
-
 Nitrobacter winogradskyi
2.3.3.1 HgCl2
-
 Nitrobacter winogradskyi
2.3.3.1 NADH no effect Nitrobacter winogradskyi
2.3.3.1 SDS strong inhibition Nitrobacter winogradskyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.1 0.027
-
 oxaloacetate
-
 Nitrobacter winogradskyi
2.3.3.1 0.41
-
 acetyl-CoA
-
 Nitrobacter winogradskyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.1 additional information no activation by metal ions Nitrobacter winogradskyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.1 45000
-
 2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE Nitrobacter winogradskyi
2.3.3.1 80000
-
 2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE Nitrobacter winogradskyi
2.3.3.1 250000
-
 gel filtration Nitrobacter winogradskyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.1 acetyl-CoA + oxaloacetate + H2O Nitrobacter winogradskyi
-
 citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.1 Nitrobacter winogradskyi
-
 strain ATCC 14123
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.1
-
 Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.1 24
-
 purified enzyme Nitrobacter winogradskyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.1 acetyl-CoA + oxaloacetate + H2O
-
 Nitrobacter winogradskyi citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.1 tetramer 2 * 45000 + 2 * 80000, alpha, beta, SDS-PAGE Nitrobacter winogradskyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.1 30 35
-
 Nitrobacter winogradskyi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.1 55
-
-
 Nitrobacter winogradskyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.1 additional information
-
 pI: 5.4 Nitrobacter winogradskyi
2.3.3.1 7.5 8
-
 Nitrobacter winogradskyi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.3.1 6 9
-
 Nitrobacter winogradskyi