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Literature summary extracted from

  • Higa, A.I.; Massarini, E.; Cazzulo, J.J.
    Purification and some properties of the citrate synthase from a marine Pseudomonas (1978), Can. J. Microbiol., 24, 215-221.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.16 AMP CS II, counteracted by NADH Pseudomonas sp.

General Stability

EC Number General Stability Organism
2.3.3.16 CS II much more stable than CS I Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 5,5'-dithiobis(2-nitrobenzoate) inactivation, half-lifes at 0.4 mM: 2.8 min for CS 1, 50 min for CS II Pseudomonas sp.
2.3.3.16 ADP CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
2.3.3.16 AMP CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
2.3.3.16 ATP CS II insensitive, CS I inhibited Pseudomonas sp.
2.3.3.16 KCl CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate Pseudomonas sp.
2.3.3.16 NADH CS II inhibited, CS II insensitive; weak inhibition, reversible by AMP, hinders the activation by AMP, CS II Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.02
-
 oxaloacetate CS I Pseudomonas sp.
2.3.3.16 0.035
-
 acetyl-CoA CS I Pseudomonas sp.
2.3.3.16 0.05
-
 oxaloacetate CS II Pseudomonas sp.
2.3.3.16 0.45
-
 acetyl-CoA CS II Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.16 KCl
-
 Pseudomonas sp.
2.3.3.16 phosphate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.16 53000
-
 2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible Pseudomonas sp.
2.3.3.16 53000
-
 6 * 53000, SDS-PAGE, CS II Pseudomonas sp.
2.3.3.16 100000
-
 gel filtration Pseudomonas sp.
2.3.3.16 100000
-
 CS I Pseudomonas sp.
2.3.3.16 300000
-
 CS II, gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Pseudomonas sp.
-
 marine, 2 enzyme forms with different molecular weight, kinetic and regulatory properties, dissociated CS I /native CS II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.16
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.16 102.7
-
 purified enzyme Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
2.3.3.16 4°C, Tris-HCl buffer, pH 7.6, 0.2 M KCl, 20% loss of activity after 1 month, only CS II Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + oxaloacetate + H2O
-
 Pseudomonas sp. citrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.16 dimer 2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible Pseudomonas sp.
2.3.3.16 hexamer 6 * 53000, SDS-PAGE, CS II Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.16 30
-
 enzyme assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.16 7.6
-
 enzyme assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.16 AMP only CS II, counteracted by NADP Pseudomonas sp.