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Literature summary extracted from

  • Leibowitz, M.J.; Soffer, R.L.
    Enzymatic modification of proteins. 3. Purification and properties of a leucyl, phenylalanyl transfer ribonucleic acid protein transferase from Escherichia coli (1970), J. Biol. Chem., 245, 2066-2073.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.6 CaCl2 20 mM, 35% inhibition Escherichia coli
2.3.2.6 Mg2+ 50 mM, 80% inhibition Escherichia coli
2.3.2.6 MnCl2 91% inhibition Escherichia coli
2.3.2.6 puromycin aminonucleoside derivative, which lacks the aminoacyl analogus structure, fails to inhibit the reaction Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.6 soluble
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.6 K+ monovalent cation required, maximal activity is 0.15 M KCl for the transfer of Phe and 0.2 M for the transfer of Leu Escherichia coli
2.3.2.6 additional information no Mg2+-requirement Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.6 Escherichia coli
-
 B
-
2.3.2.6 Escherichia coli B / ATCC 11303
-
 B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.6
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.6 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.3.2.6 -20°C, stable for at least 2 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.6 L-leucyl-tRNALeu + protein acceptor protein: bovine serum albumin Escherichia coli tRNALeu + L-leucyl-protein
-
 ?
2.3.2.6 L-leucyl-tRNALeu + protein incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli tRNALeu + L-leucyl-protein
-
 ?
2.3.2.6 L-leucyl-tRNALeu + protein acceptor protein: bovine serum albumin Escherichia coli B / ATCC 11303 tRNALeu + L-leucyl-protein
-
 ?
2.3.2.6 L-leucyl-tRNALeu + protein incorporation of Leu into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli B / ATCC 11303 tRNALeu + L-leucyl-protein
-
 ?
2.3.2.6 L-phenylalanyl-tRNA + acceptor protein incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli tRNA + L-phenylalanyl-protein
-
 ?
2.3.2.6 L-phenylalanyl-tRNA + acceptor protein incorporation of Phe into the peptide linkage with the amino-terminal aspartic acid of albumin Escherichia coli B / ATCC 11303 tRNA + L-phenylalanyl-protein
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.6 60
-
 t1/2: 1.5 min, complete inactivation after 8.5 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.6 7.6 8.2 transfer of Leu Escherichia coli
2.3.2.6 8.2 8.6 transfer of Phe Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.6 7 9 about 80% of maximal activity at pH 7.0 and 9.0 Escherichia coli