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Literature summary extracted from

  • Ikeda, Y.; Fujii, J.; Taniguchi, N.; Meister, A.
    Expression of an active glycosylated human gamma-glutamyl transpeptidase mutant that lacks a membrane anchor domain (1995), Proc. Natl. Acad. Sci. USA, 92, 126-130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 expression of wild-type and mutant lacking the putative signal sequence/anchor domain, amino acid 1-27, in Spodoptera frugiperda Sf21 cells via baculovirus infection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 brefeldin A inhibition of recombinant mutant enzyme secretion into cell culture medium from Sf21 cells, accumulation in the cells Homo sapiens
2.3.2.2 Urea complete inactivation of wild-type and mutant at 6 M, low activity at 4 M Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 1.5
-
 L-Glu-4-nitroanilide recombinant wild-type Homo sapiens
2.3.2.2 2.1
-
 L-Glu-4-nitroanilide recombinant mutant enzyme Homo sapiens
2.3.2.2 2.9 2.96 glycylglycine recombinant wild-type enzyme Homo sapiens
2.3.2.2 3.4
-
 glycylglycine recombinant mutant enzyme Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 extracellular mutant lacking the membrane anchor domain, secretion into the medium of recombinant Sf21 cell culture Homo sapiens
-
-
2.3.2.2 membrane wild-type, membrane-bound Homo sapiens 16020
-
2.3.2.2 microsome recombinant wild-type in Sf21 cells Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 24000
-
 x * 43000-44000 + x * 24000, wild-type and mutant enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein recombinant wild-type and mutant Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 recombinant wild-type and mutants from Sf21 insect cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 440
-
 purified recombinant wild-type enzyme Homo sapiens
2.3.2.2 450
-
 purified recombinant mutant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+
-
 Homo sapiens peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Homo sapiens 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 L-Glu-4-nitroanilide + glutathione
-
 Homo sapiens 4-nitroaniline + 5-L-glutamyl-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 ? x * 43000-44000 + x * 24000, wild-type and mutant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8
-
 assay at Homo sapiens