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Literature summary extracted from

  • Moriguchi, M.; Yamada, M.; Suenaga, S.; Tanaka, H.; Wakasugi, A.; Hatanaka, S.I.
    Partial purification and properties of gamma-glutamyltranspeptidase from mycelia of Morchella esculenta (1986), Arch. Microbiol., 144, 15-19.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 Maleate no stimulation Morchella esculenta
2.3.2.2 additional information phosphate, arsenate, carbonate Morchella esculenta

General Stability

EC Number General Stability Organism
2.3.2.2 dilution inactivates Morchella esculenta
2.3.2.2 glycerol, 5% v/v, stabilizes Morchella esculenta

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 L- or D-Serine/borate
-
 Morchella esculenta
2.3.2.2 p-chloromercuribenzoate no inhibition Morchella esculenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information hydrolysis data Morchella esculenta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ not hydrolase Morchella esculenta
2.3.2.2 K+ not hydrolase Morchella esculenta
2.3.2.2 Mg2+ not hydrolase Morchella esculenta
2.3.2.2 additional information
-
 Morchella esculenta
2.3.2.2 Na+ not hydrolase Morchella esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 102000
-
 isoform III, gel filtration Morchella esculenta
2.3.2.2 155000
-
 isoform I, gel filtration Morchella esculenta
2.3.2.2 219000
-
 isoform II, gel filtration Morchella esculenta

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Morchella esculenta
-
 3 isoforms I, II and III
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein
-
 Morchella esculenta

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 partial, 3 isoforms Morchella esculenta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 mycelium
-
 Morchella esculenta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 additional information
-
-
 Morchella esculenta

Storage Stability

EC Number Storage Stability Organism
2.3.2.2 -20°C, crude, at least 1 year Morchella esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: hydrolase reaction with H2O as acceptor Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donors are Glu, alpha-L-Glu-L-Ala Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ concurrent reaction: autotranspeptidation with another donor molecule as acceptor Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ Glu-donor is GSH Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor: glutathione disulfide Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ donor specificity, overview Morchella esculenta peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-aspartate
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-aspartate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-cysteine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-cysteine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-isoleucine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-isoleucine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-lysine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-lysine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-phenylalanine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-phenylalanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-proline
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-serine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-threonine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-threonine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-tryptophan
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-tryptophan
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-valine
-
 Morchella esculenta 4-nitroaniline + 5-L-glutamyl-L-valine
-
 ?
2.3.2.2 5-L-glutamyl-cis-3-amino-L-proline + acceptor
-
 Morchella esculenta 5-L-glutamyl-acceptor + cis-3-amino-L-proline
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 8.2
-
 transpeptidation, isoform II and III Morchella esculenta
2.3.2.2 8.6
-
 transpeptidation, isoform I Morchella esculenta
2.3.2.2 8.6 9 hydrolase reaction, isoform III Morchella esculenta
2.3.2.2 9
-
 hydrolase reaction, isoform II Morchella esculenta
2.3.2.2 9 9.2 hydrolase reaction, isoform I Morchella esculenta