EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.2.2 | ammonium sulfate precipitation from 20 mM Tris-HCl, pH 8.0, 14.2 mg/ml protein, refrigerator, 1 week | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.2 | 1-chloro-3-tosylamido-7-amino-2-heptanone | weak | Escherichia coli | |
2.3.2.2 | 6-diazo-5-oxo-L-norleucine | - |
Escherichia coli | |
2.3.2.2 | Hg2+ | hydrolase, not transferase reaction | Escherichia coli | |
2.3.2.2 | iodoacetate | weak | Escherichia coli | |
2.3.2.2 | L- or D-Serine/borate | L-serine/borate, not D-serine/borate | Escherichia coli | |
2.3.2.2 | L-(alphaS,5S)-alpha-Amino-3-chloro-4,5-dihydro-5-isoxazole acetic acid | i.e. AT-125; strong, irreversible, 1 mM | Escherichia coli | |
2.3.2.2 | L-1-tosylamido-2-phenylethylchloromethylketone | - |
Escherichia coli | |
2.3.2.2 | L-alanine | - |
Escherichia coli | |
2.3.2.2 | L-azaserine | - |
Escherichia coli | |
2.3.2.2 | L-glutamine | not D- | Escherichia coli | |
2.3.2.2 | additional information | - |
Escherichia coli | |
2.3.2.2 | N-ethylmaleimide | weak | Escherichia coli | |
2.3.2.2 | p-chloromercuribenzoate | no inhibition | Escherichia coli | |
2.3.2.2 | PMSF | weak | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.2 | 0.029 | 0.035 | L-cystine | hydrolase reaction | Escherichia coli | |
2.3.2.2 | 0.029 | 0.035 | L-cystine | glutathione | Escherichia coli | |
2.3.2.2 | 0.035 | - |
glutathione | - |
Escherichia coli | |
2.3.2.2 | 0.035 | - |
L-Glu-4-nitroanilide | - |
Escherichia coli | |
2.3.2.2 | 0.068 | - |
L-Glu-4-nitroanilide | hydrolase reaction | Escherichia coli | |
2.3.2.2 | 210 | - |
L-Arg | - |
Escherichia coli | |
2.3.2.2 | 590 | - |
glycylglycine | - |
Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.2.2 | periplasm | - |
Escherichia coli | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 22000 | - |
1 * 39200 + 1 * 22000, isoform A, SDS-PAGE | Escherichia coli |
2.3.2.2 | 22000 | - |
1 * 38600 + 1 * 22000, isoform B, SDS-PAGE | Escherichia coli |
2.3.2.2 | 38600 | - |
1 * 38600 + 1 * 22000, isoform B, SDS-PAGE | Escherichia coli |
2.3.2.2 | 39200 | - |
1 * 39200 + 1 * 22000, isoform A, SDS-PAGE | Escherichia coli |
2.3.2.2 | 57000 | - |
isoform B, gel filtration | Escherichia coli |
2.3.2.2 | 58000 | - |
isoform A, gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.2 | Escherichia coli | - |
K-12, strain MG1655 | - |
2.3.2.2 | Escherichia coli | - |
2 isozymes A and B | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.2 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 1.4 | - |
purified isozymes A and B | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: hydrolase reaction with H2O as acceptor | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is 5-L-Glu-4-nitroanilide | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | acceptors are amino acids or dipeptides | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | isozyme A prefers basic and aromatic amino acids as acceptors | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | concurrent reaction: autotranspeptidation with another donor molecule as acceptor | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | Glu-donor is GSH | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | (5-L-glutamyl)-peptide + acceptor + H+ | donor specificity, overview | Escherichia coli | peptide + 5-L-glutamyl amino acid | - |
? | |
2.3.2.2 | 5-L-glutamyl-4-nitroanilide + glycylglycine | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamylglycylglycine | - |
? | |
2.3.2.2 | 5-L-glutamyl-4-nitroanilide + L-asparagine | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamyl-L-asparagine | - |
? | |
2.3.2.2 | 5-L-glutamyl-4-nitroanilide + L-cystine | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamyl-L-cystine | - |
? | |
2.3.2.2 | glutathione + acceptor | acceptor: amino acids | Escherichia coli | cysteinylglycine + 5-L-glutamyl-acceptor | - |
? | |
2.3.2.2 | GSH + L-Arg | - |
Escherichia coli | ? | - |
? | |
2.3.2.2 | L-Glu-4-nitroanilide + glutathione | - |
Escherichia coli | 4-nitroaniline + 5-L-glutamyl-glutathione | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.2 | dimer | 1 * 39200 + 1 * 22000, isoform A, SDS-PAGE | Escherichia coli |
2.3.2.2 | dimer | 1 * 38600 + 1 * 22000, isoform B, SDS-PAGE | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 37 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 45 | - |
12% loss of activity within 15 min | Escherichia coli |
2.3.2.2 | 48 | - |
t1/2: 15 min | Escherichia coli |
2.3.2.2 | 50 | - |
92% loss of activity within 15 min | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.2.2 | 8.8 | - |
transpeptidation | Escherichia coli |
2.3.2.2 | 9.5 | - |
hydrolase reaction | Escherichia coli |