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Literature summary extracted from

  • Karkowsky, A.M.; Bergamini, M.V.W.; Orlowski, M.
    Kinetic studies of sheep kidney gamma-glutamyl transpeptidase (1976), J. Biol. Chem., 251, 4736-4743.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 Maleate 1.6fold Ovis aries
2.3.2.2 additional information no effect by phthalate, pyruvate and fumarate Ovis aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 5,5'-dithiobis(2-nitrobenzoate)
-
 Ovis aries
2.3.2.2 Ca2+ no inhibition Ovis aries
2.3.2.2 Co2+ not affected Ovis aries
2.3.2.2 EDTA weak Ovis aries
2.3.2.2 iodoacetamide
-
 Ovis aries
2.3.2.2 iodoacetate weak Ovis aries
2.3.2.2 Mg2+ no inhibition Ovis aries
2.3.2.2 additional information not affected by Ni2+, K+, phtalate, pyruvate and fumarate Ovis aries
2.3.2.2 N-ethylmaleimide
-
 Ovis aries
2.3.2.2 Na+ not affected Ovis aries
2.3.2.2 p-chloromercuribenzoate
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information kinetic study Ovis aries
2.3.2.2 2.9
-
 L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate hydrolase reaction Ovis aries

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.2 Ca2+ no effect Ovis aries
2.3.2.2 K+ no effect Ovis aries
2.3.2.2 Mg2+ no effect Ovis aries
2.3.2.2 additional information not affected by Co2+ and Ni2+ Ovis aries
2.3.2.2 Na+ no effect Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Ovis aries
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Ovis aries
a
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid kinetic study Ovis aries
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 kidney
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Ovis aries peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + H2O hydrolase reaction Ovis aries 4-nitroaniline + L-glutamate
-
 ir
2.3.2.2 glutathione + glutathione autotranspeptidation Ovis aries L-cysteinylglycine + 5-glutamyl-glutathione
-
 ?
2.3.2.2 glutathione + H2O hydrolase reaction, concurrent to (auto-)transpeptidation Ovis aries L-cysteinylglycine + L-glutamate
-
 ?
2.3.2.2 L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor acceptor specificity, overview Ovis aries L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
 ?
2.3.2.2 L-alpha-methyl-5-glutamyl-L-alpha-aminobutyrate + acceptor hydrolysis in the absence of acceptor, methyl group at alpha-C prevents autotranspeptidation Ovis aries L-alpha-methyl-5-glutamyl acceptor + L-2-aminobutyrate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Ovis aries