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Literature summary extracted from

  • Yasumoto, K.; Iwami, K.; Fushiki, T.; Mitsuda, H.
    Purification and enzymatic properties of gamma-glutamyltransferase from bovine colostrum (1978), J. Biochem., 84, 1227-1236.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.2 Maleate stimulates hydrolase activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.2 Acetazolamide Maleate
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.2 membrane
-
 Bos taurus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.2 25000
-
 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
2.3.2.2 55000
-
 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus
2.3.2.2 80000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bos taurus
-
 Holstein breed
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.2 glycoprotein sialoglycoprotein Bos taurus
2.3.2.2 glycoprotein both subunits are glycoproteins Bos taurus
2.3.2.2 glycoprotein 20% carbohydrate Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.2 colostrum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 407
-
 purified enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ best donor: glutamate Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptors are amino acids or dipeptides Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 (5-L-glutamyl)-peptide + acceptor + H+ acceptor specificity, overview Bos taurus peptide + 5-L-glutamyl amino acid
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate beta-enantiomer of aminobutyrate Bos taurus 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + (S)-2-aminobutyrate alpha-enantiomer of aminobutyrate Bos taurus 4-nitroaniline + N-[(1S)-1-carboxypropyl]-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide
-
 Bos taurus 5-L-glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycine 19% activity the rate of the reaction with glycylglycine, glycyl-tri- to hexapeptides can act as acceptors Bos taurus 4-nitroaniline + 5-L-glutamyl-glycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + glycylglycine best acceptor Bos taurus 4-nitroaniline + 5-L-glutamylglycylglycine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-alanine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-alanine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamate
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamate
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-glutamine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-glutamine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-methionine best acceptor Bos taurus 4-nitroaniline + 5-L-glutamyl-L-methionine
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-proline
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-proline
-
 ?
2.3.2.2 5-L-glutamyl-4-nitroanilide + L-serine
-
 Bos taurus 4-nitroaniline + 5-L-glutamyl-L-serine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 dimer 1 * 55000 + 1 * 25000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 additional information
-
 pI: 3.85 Bos taurus
2.3.2.2 8.5
-
 with acceptor glycylglycine Bos taurus
2.3.2.2 9
-
 broad Bos taurus
2.3.2.2 9
-
 without acceptor Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 7.3 8.5 about half-maximal activity at pH 7.3, maximal activity at pH 8.5 Bos taurus