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Literature summary extracted from

  • Kwak, S.J.; Kim, S.Y.; Kim, Y.S.; Song, K.Y.; Kim, I.G.; Park, S.C.
    Isolation and characterization of brain-specific transglutaminases from rat (1998), Exp. Mol. Med., 30, 177-185.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.2.13 brain transglutaminases NI and NI, very unstable Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.17
-
 putrescine brain transglutaminase NII Rattus norvegicus
2.3.2.13 0.28
-
 putrescine brain transglutaminase NI Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ brain transglutaminase NI, maximal activity at 0.1 mM, transglutaminase NII, maximal activity at 0.01 mM, inhibition above Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 29000
-
 x * 29000, brain transglutaminse NII, SDS-PAGE Rattus norvegicus
2.3.2.13 45000
-
 x * 45000, brain transglutaminase NI, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 brain transglutaminases NI and NII, DEAE-Sephacel, CM-cellulose, Q-Sepharose, FPLC gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 brain brain specific transglutaminases NI and NII Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 brain transglutaminase NI, 4°C, 2 weeks, complete loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 45000, brain transglutaminase NI, SDS-PAGE Rattus norvegicus
2.3.2.13 ? x * 29000, brain transglutaminse NII, SDS-PAGE Rattus norvegicus