EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.13 | EDTA | 5 mM; reversible | Physarum polycephalum | |
2.3.2.13 | EGTA | 2 mM, irreversible | Physarum polycephalum | |
2.3.2.13 | N-ethylmaleimide | 0.1 mM | Physarum polycephalum | |
2.3.2.13 | p-chloromercuribenzoate | 0.1 mM | Physarum polycephalum | |
2.3.2.13 | putrescine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum | |
2.3.2.13 | spermidine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum | |
2.3.2.13 | spermine | 0.1-0.2 mM, substrate inhibition | Physarum polycephalum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.2.13 | 0.0214 | - |
spermidine | - |
Physarum polycephalum | |
2.3.2.13 | 0.0317 | - |
spermine | - |
Physarum polycephalum | |
2.3.2.13 | 0.049 | - |
putrescine | - |
Physarum polycephalum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.2.13 | spheroplast | - |
Physarum polycephalum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.2.13 | Ca2+ | requirement | Physarum polycephalum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.2.13 | 39600 | - |
2 * 39600, SDS-PAGE | Physarum polycephalum |
2.3.2.13 | 77000 | - |
gel filtration | Physarum polycephalum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.13 | protein-bound gamma-glutamine + alkylamine | Physarum polycephalum | actin is probably the major endogenous substrate | protein N5-alkylglutamine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.13 | Physarum polycephalum | - |
slime mould, strain M3cV | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.13 | 15% polyethylene glycol, DEAE-cellulos, isoelectric focusing | Physarum polycephalum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.2.13 | 0.005 | - |
- |
Physarum polycephalum |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.2.13 | -20°C, partially purified, 3 years | Physarum polycephalum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.13 | protein-bound gamma-glutamine + alkylamine | actin is probably the major endogenous substrate | Physarum polycephalum | protein N5-alkylglutamine + NH3 | - |
? | |
2.3.2.13 | putrescine + bovine muscle actin | preferred substrate | Physarum polycephalum | ? | - |
? | |
2.3.2.13 | putrescine + casein | - |
Physarum polycephalum | ? | - |
? | |
2.3.2.13 | putrescine + N,N'-dimethylcasein | spermine can replace putrescine | Physarum polycephalum | ? | - |
? | |
2.3.2.13 | putrescine + N,N'-dimethylcasein | diaminopropane and cadaverine can replace putrescine | Physarum polycephalum | ? | - |
? | |
2.3.2.13 | putrescine + N,N'-dimethylcasein | spermidine can replace putrescine | Physarum polycephalum | ? | - |
? | |
2.3.2.13 | spermidine + N,N'-dimethylcasein | - |
Physarum polycephalum | ? | - |
? | |
2.3.2.13 | spermine + N,N'-dimethylcasein | - |
Physarum polycephalum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.2.13 | dimer | 2 * 39600, SDS-PAGE | Physarum polycephalum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.2.13 | 35 | - |
assay at | Physarum polycephalum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.2.13 | additional information | - |
pI: 6.1 | Physarum polycephalum |